IHC (Immunohistochemisry)
(GPX4/MCSP Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit GPX4/MCSP Polyclonal Antibody | anti-GPX4 antibody
GPX4/MCSP Rabbit anti-Human Polyclonal Antibody
Gene Names
GPX4; MCSP; PHGPx; snGPx; snPHGPx
Reactivity
Mouse, Rat, Human
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Immunoaffinity purified
Synonyms
GPX4/MCSP, Antibody; GPX4/MCSP Rabbit anti-Human Polyclonal Antibody; IHC-plus GPX4/MCSP Antibody; GPX4; GPx-4; MCSP; SnPHGPx; Phospholipid hydroperoxidase; SnGPx; Glutathione peroxidase 4; GSHPx-4; PHGPx; anti-GPX4 antibody
Host
Rabbit
Reactivity
Mouse, Rat, Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of GPx-4 protein.
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, pH7.3, 0.01% Sodium Azide, 30% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GPX4 antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry)
Target
Human GPX4/MCSP
Immunogen
Recombinant full length human GPx-4.
Conjugation
Unconjugated
Preparation and Storage
Aliquot and store at -20 degree C for 1 year. Avoid freeze/thaw cycles.
IHC (Immunohistochemisry)
(GPX4/MCSP Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemisry)
(GPX4/MCSP Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(GPX4/MCSP Antibody-Western blot analysis of GPx-4 expression in mouse kidney (A) whole cell lysates.)
WB (Western Blot)
(GPX4/MCSP Antibody-Western blot analysis of GPx-4 expression in mouse kidney (A) whole cell lysates.)
ICC (Immunocytochemistry)
(GPX4/MCSP Antibody-Immunofluorescent analysis of GPx-4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
ICC (Immunocytochemistry)
(GPX4/MCSP Antibody-Immunofluorescent analysis of GPx-4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-GPX4 antibody
MCSP antibody is an unconjugated rabbit polyclonal antibody to MCSP (GPX4) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,175 Da
NCBI Official Full Name
phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform A
NCBI Official Synonym Full Names
glutathione peroxidase 4 (phospholipid hydroperoxidase)
NCBI Official Symbol
GPX4
NCBI Official Synonym Symbols
MCSP; PHGPx; snGPx; snPHGPx
NCBI Protein Information
phospholipid hydroperoxide glutathione peroxidase, mitochondrial; GPx-4; GSHPx-4; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase
UniProt Protein Name
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
UniProt Gene Name
GPX4
UniProt Synonym Gene Names
PHGPx; GPx-4; GSHPx-4
UniProt Entry Name
GPX4_HUMAN
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Product Notes
The GPX4 gpx4 (Catalog #AAA162722) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPX4/MCSP Rabbit anti-Human Polyclonal Antibody reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPX4/MCSP can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the GPX4 gpx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPX4/MCSP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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