Mouse anti-Human HLA-DP/-DQ/-DR Monoclonal Antibody | anti-HLA-DP/-DQ/-DR antibody

HLA-DP/-DQ/-DR (MHC II)

Cat. Num. AAA23889

• Gene Names: HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry
• Synonyms: HLA-DP/-DQ/-DR, Antibody; HLA-DP/-DQ/-DR (MHC II); HLA-DPB1; HLA-DQA1; HLA-DQB1; HLA-DRA; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5;; anti-HLA-DP/-DQ/-DR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CR3/43
• Specificity: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36kDa) and a chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 258

• Applicable Applications for anti-HLA-DP/-DQ/-DR antibody: Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: ELISA: (For coating use Ab at 2-4ug/ml, order Ab without BSA); Flow Cytometry: (1-2ug/ million cells); Immunofluorescence: (1-2ug/ml); Western Blot: (1-2ug/ml); Immunohistochemistry (Formalin-fixed): (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes); ; Optimal dilution for a specific application should be determined.
• Human Chromosome Location: 6p21.3 (HLA-DP & HLA-DR)
• Immunogen: Cells from human tonsil
• Hu-chromosome Location: 6p21.3 (HLA-DP & HLA-DR)
• Positive Control: Raji cells. Tonsil or lymph node
• Cellular Localization: Cell Surface
• Positive Control: Ramos, Raji cells. Tonsil, Spleen or lymph node.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

WB (Western Blot)

(Western Blot Analysis of Human Spleen cell lysate using HLA-Pan Mouse Monoclonal Antibody (CR3/43).)

WB (Western Blot)

(Western Blot Analysis of Ramos cell lysate using HLA-Pan Mouse Monoclonal Antibody (CR3/43).)

SDS-PAGE

(SDS-PAGE Analysis Purified HLA-Pan Mouse Monoclonal Antibody (CR3/43). Confirmation of Purity and Integrity of Antibody.)

IF (Immunofluorescence)

(Immunofluorescent staining of Raji cells. HLA- Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red))

FCM (Flow Cytometry)

(Flow Cytometric Analysis of Human Raji cells using HLA-Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with HLA- Pan Mouse Monoclonal Antibody (CR3/43).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with HLA- Pan Mouse Monoclonal Antibody (CR3/43).)

References

Gatter et al. 1982. J Clin Pathol. 35(11):1253-67. Gatter et al. 1982. Semin Oncol. 9(4):517-25.

NCBI and Uniprot Product Information

• NCBI GI #: 24797076
• NCBI GeneID: 3115
• NCBI Accession #: NP_002112.3
• NCBI GenBank Nucleotide #: NM_002121.5
• UniProt Accession #: P04440; A0PFJ7; A5I886; A8YPB3; B5U8B4; B7VF80; B7VF87; B8ZX68; B8ZYT0; B9W5S8; B9W6F7; B9W6F9
• Molecular Weight: 36kDa (alpha chain); 27kDa (beta chain)
• NCBI Official Full Name: HLA class II histocompatibility antigen, DP beta 1 chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DP beta 1
• NCBI Official Symbol: HLA-DPB1
• NCBI Official Synonym Symbols: DPB1; HLA-DP; HLA-DPB; HLA-DP1B
• NCBI Protein Information: HLA class II histocompatibility antigen, DP beta 1 chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DP beta 1 chain
• UniProt Gene Name: HLA-DPB1
• UniProt Synonym Gene Names: HLA-DP1B