IF (Immunofluorescence)
(Immunofluorescent analysis of HP1 gamma staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Mouse HP1 gamma Monoclonal Antibody | anti-HP1gamma antibody
Anti-HP1 gamma Antibody
Gene Names
CBX3; HECH; HP1-GAMMA; HP1Hs-gamma
Reactivity
Human, Mouse, Rat, Monkey, Hamster
Applications
Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
HP1 gamma, Antibody; Anti-HP1 gamma Antibody; anti-HP1gamma antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey, Hamster
Clonality
Monoclonal
Specificity
Recognizes endogenous levels of HP1 gamma protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH7.3.
Applicable Applications for anti-HP1gamma antibody
IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Purified recombinant human HP1-gamma protein fragments expressed in E coli.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of HP1 gamma staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HP1 gamma staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of HP1 gamma staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.104). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of HP1 gamma staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.104). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of HP1 gamma expression in Hela (A), NIH3T3 (B), C6 (C), COS7 (D), CHOK1 (E) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 21 kD))
WB (Western Blot)
(Western blot analysis of HP1 gamma expression in Hela (A), NIH3T3 (B), C6 (C), COS7 (D), CHOK1 (E) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 21 kD))
Related Product Information for anti-HP1gamma antibody
Mouse monoclonal antibody to HP1 gamma
Product Categories/Family for anti-HP1gamma antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,811 Da
NCBI Official Full Name
chromobox protein homolog 3
NCBI Official Synonym Full Names
chromobox homolog 3
NCBI Official Symbol
CBX3
NCBI Official Synonym Symbols
HECH; HP1-GAMMA; HP1Hs-gamma
NCBI Protein Information
chromobox protein homolog 3; HP1 gamma homolog; modifier 2 protein; heterochromatin-like protein 1; heterochromatin protein HP1 gamma; heterochromatin protein 1 homolog gamma; chromobox homolog 3 (HP1 gamma homolog, Drosophila)
UniProt Protein Name
Chromobox protein homolog 3
UniProt Gene Name
CBX3
UniProt Synonym Gene Names
HP1 gamma
UniProt Entry Name
CBX3_HUMAN
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Product Notes
The HP1gamma cbx3 (Catalog #AAA265822) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-HP1 gamma Antibody reacts with Human, Mouse, Rat, Monkey, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's HP1 gamma can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HP1gamma cbx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HP1 gamma, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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