DB (Dot Blot)
(HP1 gamma phospho Ser93 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of HP1 gamma phospho Ser93 pAb for HP1 gamma phospho Ser93. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with HP1 gamma phospho Ser93 pAb at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified HP1 gamma peptide. Lane 2: phospho Ser93 HP1 gamma peptide. Lane 3: phospho Ser92 HP1 alpha peptide. Lane 4: phospho Ser89 HP1 beta peptide.)
Rabbit anti-Human HP1 gamma phospho Ser93 Polyclonal Antibody | anti-HP1gamma antibody
HP1 gamma phospho Ser93 antibody (pAb)
Gene Names
CBX3; HECH; HP1-GAMMA; HP1Hs-gamma
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
HP1 gamma phospho Ser93, Antibody; HP1 gamma phospho Ser93 antibody (pAb); HECH; CBX3; HP1Hs-gamma; anti-HP1gamma antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-HP1gamma antibody
WB (Western Blot)
Conjugation
Phosphorylated
Immunogen
This HP1 gamma phospho Ser93 antibody was raised against a peptide containing phospho Ser93 of human HP1 gamma.
Abs Residue
Ser
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(HP1 gamma phospho Ser93 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of HP1 gamma phospho Ser93 pAb for HP1 gamma phospho Ser93. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with HP1 gamma phospho Ser93 pAb at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified HP1 gamma peptide. Lane 2: phospho Ser93 HP1 gamma peptide. Lane 3: phospho Ser92 HP1 alpha peptide. Lane 4: phospho Ser89 HP1 beta peptide.)
DB (Dot Blot)
(HP1 gamma phospho Ser93 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of HP1 gamma phospho Ser93 pAb for HP1 gamma phospho Ser93. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with HP1 gamma phospho Ser93 pAb at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified HP1 gamma peptide. Lane 2: phospho Ser93 HP1 gamma peptide. Lane 3: phospho Ser92 HP1 alpha peptide. Lane 4: phospho Ser89 HP1 beta peptide.)
WB (Western Blot)
(HP1 gamma phospho Ser93 pAb tested by Western blot. HeLa nuclear extract (20 ug per lane) was probed with HP1 gamma phospho Ser93 pAb (1:500 dilution). Lane 1: no treatment. Lane 2: cells treated with colcemid.)
WB (Western Blot)
(HP1 gamma phospho Ser93 pAb tested by Western blot. HeLa nuclear extract (20 ug per lane) was probed with HP1 gamma phospho Ser93 pAb (1:500 dilution). Lane 1: no treatment. Lane 2: cells treated with colcemid.)
Related Product Information for anti-HP1gamma antibody
Short Description: Our HP1 gamma phospho Ser93 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: Heterochromatin Protein 1 gamma (HP1 gamma) is one of the mammalian homologues of Drosophila HP1, first identified through its role in the maintenance of regions of the genome in a heterochromatic state. HP1 gamma contains a Chromo domain and a Chromo shadow domain. Through the Chromo domain, HP1 proteins are recruited to regions of chromatin that exhibit methylation of lysine 9 of histone H3. Phosphorylation of serine 93 of HP1 gamma increases during mitosis.
Background: Heterochromatin Protein 1 gamma (HP1 gamma) is one of the mammalian homologues of Drosophila HP1, first identified through its role in the maintenance of regions of the genome in a heterochromatic state. HP1 gamma contains a Chromo domain and a Chromo shadow domain. Through the Chromo domain, HP1 proteins are recruited to regions of chromatin that exhibit methylation of lysine 9 of histone H3. Phosphorylation of serine 93 of HP1 gamma increases during mitosis.
Product Categories/Family for anti-HP1gamma antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,811 Da
NCBI Official Full Name
chromobox protein homolog 3
NCBI Official Synonym Full Names
chromobox homolog 3
NCBI Official Symbol
CBX3
NCBI Official Synonym Symbols
HECH; HP1-GAMMA; HP1Hs-gamma
NCBI Protein Information
chromobox protein homolog 3; HP1 gamma homolog; chromobox homolog 3 (HP1 gamma homolog, Drosophila); heterochromatin protein 1 homolog gamma; heterochromatin protein HP1 gamma; heterochromatin-like protein 1; modifier 2 protein
UniProt Protein Name
Chromobox protein homolog 3
UniProt Gene Name
CBX3
UniProt Synonym Gene Names
HP1 gamma
UniProt Entry Name
CBX3_HUMAN
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Product Notes
The HP1gamma cbx3 (Catalog #AAA59908) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HP1 gamma phospho Ser93 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HP1 gamma phospho Ser93 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the HP1gamma cbx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HP1 gamma phospho Ser93, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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