IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774))
Mouse HSP27 (Heat Shock Protein 27) Monoclonal Antibody | anti-HSPB1 antibody
HSP27 (Heat Shock Protein 27) Mouse Monoclonal Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human, Chimpanzee, Sheep, Rat, Mouse, Chicken
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
HSP27 (Heat Shock Protein 27), Antibody; HSP27 (Heat Shock Protein 27) Mouse Monoclonal Antibody; Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27); anti-HSPB1 antibody
Host
Mouse
Reactivity
Human, Chimpanzee, Sheep, Rat, Mouse, Chicken
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
HSPB1/774
Specificity
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced -p29- and -24K- protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Sequence Length
205
Applicable Applications for anti-HSPB1 antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Predominantly Cytoplasmic with some nuclear
Immunogen
Recombinant human HSPB1 protein
Hu-Chromosome Location
7q11.23
Positive Control
~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774))
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774))
References
Georgopoulos, C. and Welch, W.J. 1993. Role of the major heat shock proteins as molecular chaperones. Annu. Rev. Cell Biol. 9: 601-634
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27kDa
NCBI Official Full Name
heat shock protein beta-1
NCBI Official Synonym Full Names
heat shock protein family B (small) member 1
NCBI Official Symbol
HSPB1
NCBI Official Synonym Symbols
CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
NCBI Protein Information
heat shock protein beta-1
UniProt Protein Name
Heat shock protein beta-1
UniProt Gene Name
HSPB1
UniProt Synonym Gene Names
HSP27; HSP28; HspB1; HSP 27; SRP27
UniProt Entry Name
HSPB1_HUMAN
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Product Notes
The HSPB1 hspb1 (Catalog #AAA62838) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSP27 (Heat Shock Protein 27) Mouse Monoclonal Antibody reacts with Human, Chimpanzee, Sheep, Rat, Mouse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's HSP27 (Heat Shock Protein 27) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the HSPB1 hspb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP27 (Heat Shock Protein 27), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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