Mouse anti-Human ISG15 Monoclonal Antibody | anti-ISG15 antibody

ISG15 Antibody (N-term)

Cat. Num. AAA290650

• Gene Names: ISG15; G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
• Reactivity: Human
• Applications: ELISA, Western Blot
• Synonyms: ISG15, Antibody; ISG15 Antibody (N-term); Ubiquitin-like protein ISG15; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP; ISG15; G1P2; UCRP; anti-ISG15 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1031CT2.8.3
• Specificity: This ISG15 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-165 amino acids from the N-terminal region of human ISG15.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 165

• Applicable Applications for anti-ISG15 antibody: ELISA, WB (Western Blot)
• Cellular Location: Cytoplasm. Secreted. Note=Exists in three distinct states: free within the cell, released into the extracellular space, or conjugated to target proteins.
• Tissue Location: Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. Expressed in neutrophils, monocytes and lymphocytes. Enhanced expression seen in pancreatic adenocarcinoma, endometrial cancer, and bladder cancer, as compared to non-cancerous tissue. In bladder cancer, the increase in expression exhibits a striking positive correlation with more advanced stages of the disease.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes : Anti-ISG15 Antibody (N-term)) at 1:8000 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-ISG15 antibody

Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include IFIT1, MX1/MxA, PPM1B, UBE2L6, UBA7, CHMP5, CHMP2A, CHMP4B and CHMP6. Can also isgylate: EIF2AK2/PKR which results in its activation, DDX58/RIG-I which inhibits its function in antiviral signaling response, EIF4E2 which enhances its cap structure-binding activity and translation- inhibition activity, UBE2N and UBE2E1 which negatively regulates their activity, IRF3 which inhibits its ubiquitination and degradation and FLNB which prevents its ability to interact with the upstream activators of the JNK cascade therby inhibiting IFNA- induced JNK signaling. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A, HIV-1 and Ebola virus. Restricts HIV-1 and ebola virus via disruption of viral budding. Inhibits the ubiquitination of HIV-1 Gag and host TSG101 and disrupts their interaction, thereby preventing assembly and release of virions from infected cells. Inhibits Ebola virus budding mediated by the VP40 protein by disrupting ubiquitin ligase activity of NEDD4 and its ability to ubiquitinate VP40. ISGylates influenza A virus NS1 protein which causes a loss of function of the protein and the inhibition of virus replication. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity.

Product Categories/Family for anti-ISG15 antibody

Crown Antibodies; Primary Antibodies; Cell Biology

References

Blomstrom D.C.,et al.J. Biol. Chem. 261:8811-8816 (1986). Reich N.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:6394-6398 (1987). Knight E. Jr.,et al.J. Biol. Chem. 263:4520-4522 (1988). Knight E. Jr.,et al.J. Biol. Chem. 263:10040-10040 (1988). Kamitani T.,et al.Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 4826774
• NCBI GeneID: 9636
• NCBI Accession #: NP_005092.1
• NCBI GenBank Nucleotide #: NM_005101.3
• UniProt Accession #: P05161; Q5SVA4; Q7Z2G2; Q96GF0
• Molecular Weight: 17888 Da
• NCBI Official Full Name: ubiquitin-like protein ISG15
• NCBI Official Synonym Full Names: ISG15 ubiquitin-like modifier
• NCBI Official Symbol: ISG15
• NCBI Official Synonym Symbols: G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
• NCBI Protein Information: ubiquitin-like protein ISG15
• UniProt Protein Name: Ubiquitin-like protein ISG15
• UniProt Gene Name: ISG15
• UniProt Synonym Gene Names: G1P2; UCRP; IP17; hUCRP
• UniProt Entry Name: ISG15_HUMAN