Mouse MAML2 (Mastermind Like Transcriptional Coactivator 2) Monoclonal Antibody | anti-MAML2 antibody

MAML2 (Mastermind Like Transcriptional Coactivator 2)

Cat. Num. AAA214565

• Gene Names: MAML2; MAM2; MAM3; MAM-3; MLL-MAML2
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: MAML2 (Mastermind Like Transcriptional Coactivator 2), Antibody; MAML2 (Mastermind Like Transcriptional Coactivator 2); Mam-2; MAML2; Mastermind-like protein 2; anti-MAML2 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: MAML2/1302
• Specificity: MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jkappa; a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1156

• Applicable Applications for anti-MAML2 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant human full-length MAML2
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 11q21
• Positive Control: MCF-7 cells. Pancreas or Placenta. Bladder or Colon Carcinoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Human Placenta stained with MAML2 Monoclonal Antibody (MAML2/1302).)

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with MAML2 Monoclonal Antibody (MAML2/1302).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with MAML2 Monoclonal Antibody (MAML2/1302).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Human Pancreas stained with MAML2 Monoclonal Antibody (MAML2/1302).)

References

Wu, L., Sun, T., Kobayashi, K., Gao, P. and Griffin, J.D. 2002. Identification of a family of mastermind-like transcriptional co-activators for mammalian Notch receptors. Mol. Cell. Biol. 22: 7688-770.

NCBI and Uniprot Product Information

• NCBI GI #: 33286444
• NCBI GeneID: 84441
• NCBI Accession #: NP_115803.1
• NCBI GenBank Nucleotide #: NM_032427.3
• UniProt Accession #: Q8IZL2; Q6AI23; Q6Y3A3; Q8IUL3; Q96JK6; A7MD26
• Molecular Weight: 125kDa
• NCBI Official Full Name: mastermind-like protein 2
• NCBI Official Synonym Full Names: mastermind like transcriptional coactivator 2
• NCBI Official Symbol: MAML2
• NCBI Official Synonym Symbols: MAM2; MAM3; MAM-3; MLL-MAML2
• NCBI Protein Information: mastermind-like protein 2
• UniProt Protein Name: Mastermind-like protein 2
• UniProt Gene Name: MAML2
• UniProt Synonym Gene Names: KIAA1819; Mam-2