MAPK10 recombinant antibody

MAPK10 Recombinant Monoclonal Antibody

Cat. Num. AAA278983

• Gene Names: MAPK10; JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: Affinity purified
• Synonyms: MAPK10, Recombinant Antibody; MAPK10 Recombinant Monoclonal Antibody; Mitogen-activated protein kinase 10 (MAP kinase 10)(MAPK 10)(EC 2.7.11.24)(MAP kinase p49 3F12)(Stress-activated protein kinase 1b)(SAPK1b)(Stress-activated protein kinase JNK3)(c-Jun N-terminal kinase 3); MAPK10; JNK3 JNK3A PRKM10 SAPK1B; MAPK10 recombinant antibody

• Host: HEK293F Cell
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 29E10
• Purity/Purification: Affinity purified
• Form/Format: Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.

• Applicable Applications for MAPK10 recombinant antibody: FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen Species: Human
• Immunogen: A synthesized peptide derived from Human MAPK10
• Conjugation: Non-conjugated
• Research Area: Cancer; Immunology; Signal transduction
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

FCM/FACS (Flow Cytometry)

(Overlay Peak curve showing PC3 cells stained with AAA278983 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)

IF (Immunofluorescence)

(Immunofluorescence staining of PC-3 cell with AAA278983 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 553-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

IHC (Immunohiostchemistry)

(IHC image of AAA278983 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)

WB (Western Blot)

(Western BlotPositive WB detected in: PC-3 whole cell lysate, Mouse brain tissueAll lanes: MAPK10 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53, 49, 32kDaObserved band size: 55 kDa)

Related Product Information for MAPK10 recombinant antibody

Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296). {ECO:0000269|PubMed:11718727, ECO:0000269|PubMed:22327296, ECO:0000269|PubMed:22441692}.

NCBI and Uniprot Product Information

• NCBI GI #: 4506081
• NCBI GeneID: 5602
• NCBI Accession #: NP_002744.1
• NCBI GenBank Nucleotide #: NM_002753.3
• UniProt Accession #: P53779; Q15707; Q49AP1; A6NFS3; A6NG28; B3KQ94
• Molecular Weight: 52,585 Da
• NCBI Official Full Name: mitogen-activated protein kinase 10 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 10
• NCBI Official Symbol: MAPK10
• NCBI Official Synonym Symbols: JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
• NCBI Protein Information: mitogen-activated protein kinase 10; MAPK 10; MAP kinase 10; MAP kinase p49 3F12; JNK3 alpha protein kinase; c-Jun N-terminal kinase 3; stress-activated protein kinase 1b; stress activated protein kinase beta; stress-activated protein kinase JNK3
• UniProt Protein Name: Mitogen-activated protein kinase 10
• UniProt Gene Name: MAPK10
• UniProt Synonym Gene Names: JNK3; JNK3A; PRKM10; SAPK1B; MAP kinase 10; MAPK 10; SAPK1b
• UniProt Entry Name: MK10_HUMAN