Rat anti-Mouse MHC Class II Monoclonal Antibody | anti-MHCClassII antibody

Anti-Mouse MHC Class II In Vivo Antibody - Low Endotoxin (M5/114.15.2)

Cat. Num. AAA197041

• Reactivity: Mouse
• Applications: Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: >95% by SDS-PAGE and HPLC; This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
• Synonyms: MHC Class II, Antibody; Anti-Mouse MHC Class II In Vivo Antibody - Low Endotoxin (M5/114.15.2); I-A/I-E MHC class II; anti-MHCClassII antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2b
• Clone Number: M5/114.15.2
• Specificity: Clone M5/114.15.2 recognizes an epitope on the mouse MHC class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. Clone M5/114.15.2 does not react with I-Af, I-Ak, or I-As.
• Purity/Purification: >95% by SDS-PAGE and HPLC; This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
• Form/Format: This M5/114.15.2 in vivo antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free.
• Concentration: >2.0 mg/ml (varies by lot)

• Applicable Applications for anti-MHCClassII antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), Blocking
• Endotoxin: <1.0 EU/mg as determined by the LAL method
• Immunogen: C57BL/6 Spleen Cells
• Aggregation: Aggregation level < 5%
• Antigen Distribution: I-Ab/d/q and I-Ed/k are expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells from mice of the I-Ab/d/q and I-Ed/k haplotypes.
• Preparation and Storage: This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Related Product Information for anti-MHCClassII antibody

MHC class II antibody, clone M5/114.15.2, recognizes the major histocompatibility complex (MHC) class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. MHC class II is constitutively expressed on professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation. MHC class II consists of two transmembrane proteins, a 35 kDa alpha (heavy) chain and 29 kDa beta (light) chain. The N-terminal alpha1 and beta1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation and can transduce signals into T cells, enhancing T cell proliferation and activity.

Product Categories/Family for anti-MHCClassII antibody

Low Endotoxin; anti-mouse