Application Data
Mouse anti-Rat Neurofilament NF-L ms Monoclonal Antibody | anti-NEFL antibody
Anti-Neurofilament L (NF-L)
Gene Names
NEFL; NFL; NF-L; NF68; CMT1F; CMT2E
Reactivity
Rat
Applications
Immunofluorescence, Western Blot
Purity
Total IgG fraction (Unpurified, concentrated culture supernatant.)
Synonyms
Neurofilament NF-L ms, Antibody; Anti-Neurofilament L (NF-L); anti-NEFL antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
DA2
Specificity
Specific for the ~68k Neurofilament L protein.
Purity/Purification
Total IgG fraction (Unpurified, concentrated culture supernatant.)
Form/Format
Liquid containing 10mM sodium azide
Sequence Length
549
Applicable Applications for anti-NEFL antibody
IF (Immunofluorescence), WB (Western Blot)
Antigen
Preparation of porcine intermediate filaments
Immunogen Information
Preparation of porcine NF-L
Immunogen Species
Pig
Reactivity Assumed Based on 100% Sequence Homology
Chicken, human, mouse
Species Reactivity Note
The antibody has been directly tested for reactivity in bovine, chicken, human, mouse and rat tissues.
Biological Significance
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2).
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Application Data
Application Data
Related Product Information for anti-NEFL antibody
Mouse monoclonal antibody
References
• Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
• Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46.
• Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
68
NCBI Official Full Name
Neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament, light polypeptide
NCBI Official Symbol
NEFL
NCBI Official Synonym Symbols
NFL; NF-L; NF68; CMT1F; CMT2E
NCBI Protein Information
neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein
UniProt Protein Name
Neurofilament light polypeptide
UniProt Gene Name
NEFL
UniProt Synonym Gene Names
NF-L
UniProt Entry Name
NFL_PIG
Similar Products
Product Notes
The NEFL nefl (Catalog #AAA72747) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Neurofilament L (NF-L) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament NF-L ms can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the NEFL nefl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament NF-L ms, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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