Mouse Neurofilament NF-L Monoclonal Antibody | anti-NF-L antibody

Neurofilament NF-L

Cat. Num. AAA76636

• Gene Names: NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
• Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
• Synonyms: Neurofilament NF-L, Antibody; Neurofilament NF-L; anti-NF-L antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Form/Format: Affinity Purified antibody; Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

• Applicable Applications for anti-NF-L antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
• Immunogen: Full length native protein purified from pig spinal cord
• Preparation and Storage: Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2-8 degree C for 1 month without detectable loss ofactivity. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of different tissue lysates using mouse mAb to NF-L dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.)

IF (Immunofluorescence)

(Immunofluorescent analysis of a section of mouse cerebellum stained with mouse mAb to NF-L dilution 1:5,000 in green, and costained with chicken pAb to FOX2 dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA.)

Related Product Information for anti-NF-L antibody

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L like MCA-6H112 are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig's disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. Much interest has recently been focused on the detection of NF-L released from neurons into blood and CSF as a surrogate marker of primarily axonal loss in a variety of types of CNS injury and degeneration.

NCBI and Uniprot Product Information

• NCBI GI #: 62511894
• NCBI GeneID: 4747
• UniProt Accession #: P07196; Q16154; Q8IU72; B9ZVN2
• Molecular Weight: 543
• NCBI Official Full Name: Neurofilament light polypeptide
• NCBI Official Synonym Full Names: neurofilament, light polypeptide
• NCBI Official Symbol: NEFL
• NCBI Official Synonym Symbols: NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
• NCBI Protein Information: neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein; protein phosphatase 1, regulatory subun
• UniProt Protein Name: Neurofilament light polypeptide
• UniProt Gene Name: NEFL
• UniProt Synonym Gene Names: NF68; NFL; NF-L
• UniProt Entry Name: NFL_HUMAN