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product-image-AAA282560_SDS_PAGE15.jpg SDS-PAGE (Recombinant Human NF-L/NEFL Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 28 kDa.)

NF-L/NEFL recombinant protein

Recombinant Human NF-L/NEFL Protein

Gene Names
NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
Purity
> 95% by SDS-PAGE.
Synonyms
NF-L/NEFL; N/A; Recombinant Human NF-L/NEFL Protein; NEFL; CMT1F; CMT2E; NF-L; NF68; NFL; PPP1R110; NF-L/NEFL recombinant protein
Ordering
Host
E coli
Purity/Purification
> 95% by SDS-PAGE.
Form/Format
Liquid
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Species
E.coli
Tags
No tag
Endotoxin
<0.1EU/ug
Reconstitution
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Preparation and Storage
Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8? for up to 1 week. Avoid repeated freeze/thaw cycles.

SDS-PAGE

(Recombinant Human NF-L/NEFL Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 28 kDa.)

product-image-AAA282560_SDS_PAGE15.jpg SDS-PAGE (Recombinant Human NF-L/NEFL Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 28 kDa.)
Related Product Information for NF-L/NEFL recombinant protein
Recombinant Human NF-L/NEFL Protein is produced by E coli expression system. The target protein is expressed with sequence (Ser60-Thr250) of human NF-L/NEFL (Accession #NP_006149.2) fused with a additional amino acid free.


Background: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. Mayadditionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Product Categories/Family for NF-L/NEFL recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament, light polypeptide
NCBI Official Symbol
NEFL
NCBI Official Synonym Symbols
NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
NCBI Protein Information
neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein; protein phosphatase 1, regulatory subun
UniProt Protein Name
Neurofilament light polypeptide
UniProt Gene Name
NEFL
UniProt Synonym Gene Names
NF68; NFL; NF-L
UniProt Entry Name
NFL_HUMAN

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Product Notes

The NF-L/NEFL nefl (Catalog #AAA282560) is a Recombinant Protein produced from E coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NF-L/NEFL, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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