IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).)
Mouse Neurofilament (NF-L) Monoclonal Antibody | anti-NEFL antibody
Neurofilament (NF-L) (Neuronal Marker) Mouse Monoclonal Antibody
Gene Names
NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
Reactivity
Human, Rat, Pig, Cow, Chicken
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
Neurofilament (NF-L), Antibody; Neurofilament (NF-L) (Neuronal Marker) Mouse Monoclonal Antibody; 68kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68; anti-NEFL antibody
Host
Mouse
Reactivity
Human, Rat, Pig, Cow, Chicken
Clonality
Monoclonal
Isotype
IgG1
Clone Number
NR-4
Specificity
This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
543
Applicable Applications for anti-NEFL antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence)
Cellular Localization
Cytoplasmic
Immunogen
Crude neurofilament preparation from porcine spinal cord
Hu-Chromosome Location
8p21.2
Positive Control
Brain, Neuroblastoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).)
References
Debus E., Weber K., Osborn M. Monoclonal antibodies specific for glial fibrillary acidic (GFA) protein and for each of the neurofilament triplet polypeptides. Differentiation 25 (2): 193-203, (1983). Angelides, K.J., et. al. 1989. J. Cell Biol. 108: 1495-1506.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68kDa
NCBI Official Full Name
neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament, light polypeptide
NCBI Official Symbol
NEFL
NCBI Official Synonym Symbols
NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
NCBI Protein Information
neurofilament light polypeptide
UniProt Protein Name
Neurofilament light polypeptide
UniProt Gene Name
NEFL
UniProt Synonym Gene Names
NF68; NFL; NF-L
UniProt Entry Name
NFL_HUMAN
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Product Notes
The NEFL nefl (Catalog #AAA62905) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neurofilament (NF-L) (Neuronal Marker) Mouse Monoclonal Antibody reacts with Human, Rat, Pig, Cow, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament (NF-L) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the NEFL nefl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament (NF-L), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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