WB (Western Blot)
(Western Blot analysis using Nucleophosmin antibodyWestern Blot showing NPM antibody used against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.)
Mouse Nucleophosmin Monoclonal Antibody | anti-NPM1 antibody
Nucleophosmin antibody
Gene Names
NPM1; B23; NPM
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
Nucleophosmin, Antibody; Nucleophosmin antibody; Monoclonal Nucleophosmin; Anti-Nucleophosmin; MGC104254; nucleolar phosphoprotein B23; NPM; nucleophosmin/nucleoplasmin family; Nucleophosmin; numatrin; B23; Anti Nucleophosmin; member 1; Nucleolar phosphoprotein B23; anti-NPM1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7H10B9
Form/Format
Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
294
Applicable Applications for anti-NPM1 antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA
Biological Significance
Nucleophosmin is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage. Nucleophosmin is upregulated in many cancer cells.
Cross-Reactivity
Human, Monkey
Immunogen
Nucleophosmin antibody was raised in Mouse using a purified recombinant fragment of human NPM expressed in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Western Blot analysis using Nucleophosmin antibodyWestern Blot showing NPM antibody used against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.)
WB (Western Blot)
(Western Blot analysis using Nucleophosmin antibodyWestern Blot showing NPM antibody used against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.)
IHC (Immunohiostchemistry)
(Immunohistochemical staining using Nucleophosmin antibodyImmunohistochemical analysis of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using NPM antibody with DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical staining using Nucleophosmin antibodyImmunohistochemical analysis of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using NPM antibody with DAB staining.)
IF (Immunofluorescence)
(Immunofluorescent staining using Western Blot analysis using Nucleophosmin antibodyConfocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.)
IF (Immunofluorescence)
(Immunofluorescent staining using Western Blot analysis using Nucleophosmin antibodyConfocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.)
Related Product Information for anti-NPM1 antibody
Mouse monoclonal Nucleophosmin antibody
Product Categories/Family for anti-NPM1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
28,400 Da
NCBI Official Full Name
nucleophosmin
NCBI Official Synonym Full Names
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
NCBI Official Symbol
NPM1
NCBI Official Synonym Symbols
B23; NPM
NCBI Protein Information
nucleophosmin
UniProt Protein Name
Nucleophosmin
UniProt Gene Name
NPM1
UniProt Synonym Gene Names
NPM; NPM
UniProt Entry Name
NPM_HUMAN
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Product Notes
The NPM1 npm1 (Catalog #AAA106340) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nucleophosmin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the NPM1 npm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleophosmin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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