IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NUMB protein using NUMB antibody)
Mouse NUMB Monoclonal Antibody | anti-NUMB antibody
NUMB antibody
Gene Names
NUMB; S171; C14orf41; c14_5527
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
NUMB antibody was purified by affinity chromatography.
Synonyms
NUMB, Antibody; NUMB antibody; Monoclonal NUMB; Anti-NUMB; Numb homolog; h-Numb; S171; Protein numb homolog; anti-NUMB antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1A7
Purity/Purification
NUMB antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
640
Applicable Applications for anti-NUMB antibody
WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Biological Significance
NUMB plays a role in the determination of cell fates during development. The NUMB protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that hasbeen shown to associate with EPS15, LNX1, and NOTCH1.
Cross-Reactivity
Human
Immunogen
NUMB antibody was raised in mouse using a full length recombinant protein of human NUMB (NP_003735) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NUMB protein using NUMB antibody)
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NUMB protein using NUMB antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NUMB protein (Right) or empty vector (Left) detected with NUMB antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NUMB protein (Right) or empty vector (Left) detected with NUMB antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant NUMB protein (red) or empty vector (blue) stained using NUMB antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant NUMB protein (red) or empty vector (blue) stained using NUMB antibody)
Related Product Information for anti-NUMB antibody
Mouse monoclonal NUMB antibody
Product Categories/Family for anti-NUMB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,396 Da
NCBI Official Full Name
protein numb homolog isoform 3
NCBI Official Synonym Full Names
numb homolog (Drosophila)
NCBI Official Symbol
NUMB
NCBI Official Synonym Symbols
S171; C14orf41; c14_5527
NCBI Protein Information
protein numb homolog
UniProt Protein Name
Protein numb homolog
UniProt Gene Name
NUMB
UniProt Synonym Gene Names
h-Numb
UniProt Entry Name
NUMB_HUMAN
Similar Products
Product Notes
The NUMB numb (Catalog #AAA107301) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NUMB can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the NUMB numb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUMB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.