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Knockout Validation
(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: NUMB knockout A431 cell lysate; Predicted MW: 71kd Observed MW: 71kd Primary Ab: 2ug/ml Rabbit Anti-Human NUMB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit anti-Human, Mouse Numb Homolog (NUMB) Polyclonal Antibody | anti-NUMB antibody
Polyclonal Antibody to Numb Homolog (NUMB)
Gene Names
NUMB; S171; C14orf41; c14_5527
Reactivity
Human, Mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Chromatography
Synonyms
Numb Homolog (NUMB), Antibody; Polyclonal Antibody to Numb Homolog (NUMB); anti-NUMB antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NUMB. It has been selected for its ability to recognize NUMB in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LAKPA HTALAPVAMP VRETNPWAHA PDAANKEIAA TCSGTEWGQS SGAASPGLFQ AGHRRTPSEA
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LAKPA HTALAPVAMP VRETNPWAHA PDAANKEIAA TCSGTEWGQS SGAASPGLFQ AGHRRTPSEA
Sequence Length
643
Applicable Applications for anti-NUMB antibody
WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Immunogen
Recombinant NUMB (Leu376~Phe482) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: NUMB knockout A431 cell lysate; Predicted MW: 71kd Observed MW: 71kd Primary Ab: 2ug/ml Rabbit Anti-Human NUMB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: NUMB knockout A431 cell lysate; Predicted MW: 71kd Observed MW: 71kd Primary Ab: 2ug/ml Rabbit Anti-Human NUMB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemisry)
(DAB staining on IHC-P. Samples: Human Tissue))
IHC (Immunohistochemisry)
(DAB staining on IHC-P. Samples: Human Tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Human A431 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Human NUMB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Human A431 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Human NUMB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Product Categories/Family for anti-NUMB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,396 Da
NCBI Official Full Name
protein numb homolog isoform 1
NCBI Official Synonym Full Names
NUMB, endocytic adaptor protein
NCBI Official Symbol
NUMB
NCBI Official Synonym Symbols
S171; C14orf41; c14_5527
NCBI Protein Information
protein numb homolog
UniProt Protein Name
Protein numb homolog
UniProt Gene Name
NUMB
UniProt Synonym Gene Names
h-Numb
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Product Notes
The NUMB numb (Catalog #AAA135057) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Numb Homolog (NUMB) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Numb Homolog (NUMB) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the NUMB numb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LAKPA HTALAPVAMP VRETNPWAHA PDAANKEIAA TCSGTEWGQS SGAASPGLFQ AGHRRTPSEA. It is sometimes possible for the material contained within the vial of "Numb Homolog (NUMB), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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