WB (Western Blot)
(Detection of human NUMB by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUMB antibody AAA211599 (lot AAA211599-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
Rabbit anti-Human NUMB Polyclonal Antibody | anti-NUMB antibody
Rabbit anti-NUMB Antibody, Affinity Purified
Gene Names
NUMB; S171; C14orf41; c14_5527
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NUMB, Antibody; Rabbit anti-NUMB Antibody, Affinity Purified; h-Numb; S171; Protein S171; numb homolog; C14orf41; c14_5527; protein numb homolog; Protein numb homolog; NUMB, endocytic adaptor protein; anti-NUMB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-NUMB antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 375 and 425
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NUMB by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUMB antibody AAA211599 (lot AAA211599-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human NUMB by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUMB antibody AAA211599 (lot AAA211599-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NUMB by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUMB antibody AAA211599 (lot AAA211599-1) used for IP at 3 ug/mg lysate. NUMB was also immunoprecipitated by rabbit anti-NUMB antibody which recognizes a downstream epitope. For blotting immunoprecipitated NUMB, AAA211599 was used at 0.1 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NUMB by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUMB antibody AAA211599 (lot AAA211599-1) used for IP at 3 ug/mg lysate. NUMB was also immunoprecipitated by rabbit anti-NUMB antibody which recognizes a downstream epitope. For blotting immunoprecipitated NUMB, AAA211599 was used at 0.1 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
Related Product Information for anti-NUMB antibody
NUMB plays an essential role in the determination of cell fate and development and appears to be especially important to sensory organogenesis and neurogenesis. NUMB is a notch1-interacting protein that antagonizes the notch1 signaling pathway. In the cell, NUMB is asymmetrically localized. The polarized distribution of NUMB in the cell is fundamental to its function of promoting asymmetric cell division for cell fate specification. Numb is also implicated to function as an endocytic protein, as it interacts with EPS15, a protein involved in endocytosis.
Product Categories/Family for anti-NUMB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,396 Da
NCBI Official Full Name
protein numb homolog isoform 1
NCBI Official Synonym Full Names
NUMB, endocytic adaptor protein
NCBI Official Symbol
NUMB
NCBI Official Synonym Symbols
S171; C14orf41; c14_5527
NCBI Protein Information
protein numb homolog
UniProt Protein Name
Protein numb homolog
UniProt Gene Name
NUMB
UniProt Synonym Gene Names
h-Numb
UniProt Entry Name
NUMB_HUMAN
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Product Notes
The NUMB numb (Catalog #AAA211599) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NUMB Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUMB can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NUMB numb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUMB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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