<
WB (Western Blot)
(Figure-8: Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 2 ug/ml on h Spleen lysate.)
Mouse anti-Human PD-L1 Monoclonal Antibody | anti-PD-L1 antibody
Monoclonal Antibody to Human PD-L1 (Clone: ABM4E54)(BSA/AZIDE Free)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
Purity
Protein G Chromatography
Synonyms
PD-L1, Antibody; Monoclonal Antibody to Human PD-L1 (Clone: ABM4E54)(BSA/AZIDE Free); CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1; anti-PD-L1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a Kappa
Clone Number
ABM4E54
Purity/Purification
Protein G Chromatography
Form/Format
Purified; 25 ug in 50 ul/100 ug in 200 ul PBS (sterile filtered), BSA, Glycerol and Sodium azide free.
Applicable Applications for anti-PD-L1 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
WB: 2-4 ug/ml
IHC: 5-10 ug/ml
FACS: 1-2 ug/ml
IHC: 5-10 ug/ml
FACS: 1-2 ug/ml
Immunogen
A partial length recombinant PDL1 protein (amino acids 18-227) was used as the immunogen for this antibody.
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months. For long-term storage; store at -20 degree C. Avoid repeated freeze and thaw cycles.
WB (Western Blot)
(Figure-8: Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 2 ug/ml on h Spleen lysate.)
WB (Western Blot)
(Figure-8: Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 2 ug/ml on h Spleen lysate.)
IHC (Immunohistochemistry)
(Figure-7: Immunohistochemical analysis of PD-L1 in Human Lung Cancer tissue using PD-L1 antibody (Clone: ABM4E54).)
IHC (Immunohistochemistry)
(Figure-7: Immunohistochemical analysis of PD-L1 in Human Lung Cancer tissue using PD-L1 antibody (Clone: ABM4E54).)
WB (Western Blot)
(Figure-6: Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 0.5 ug/ml on (1) HepG2, (2) SKBR3, (3) A431, (4) THP1, (5) NCCIT, (6) PC3, (7) PANC-1, (8) U87 and (9) KATO-111 lysates.)
WB (Western Blot)
(Figure-6: Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 0.5 ug/ml on (1) HepG2, (2) SKBR3, (3) A431, (4) THP1, (5) NCCIT, (6) PC3, (7) PANC-1, (8) U87 and (9) KATO-111 lysates.)
IHC (Immunohistochemistry)
(Figure-5: Immunohistochemical analysis of PD-L1 in Human Tonsil tissue using PD-L1 antibody (Clone: ABM4E54) at 5 ug/ml.)
IHC (Immunohistochemistry)
(Figure-5: Immunohistochemical analysis of PD-L1 in Human Tonsil tissue using PD-L1 antibody (Clone: ABM4E54) at 5 ug/ml.)
WB (Western Blot)
(Figure-4: Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 2 ug/ml on (1) A549, (2) MCF-7, (3) 293, (4) HCT-116, (5) Saos2 and (6) Hela lysates.)
WB (Western Blot)
(Figure-4: Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 2 ug/ml on (1) A549, (2) MCF-7, (3) 293, (4) HCT-116, (5) Saos2 and (6) Hela lysates.)
WB (Western Blot)
(Figure-3: Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 0.5 ug/ml on Recombinat lysates.)
WB (Western Blot)
(Figure-3: Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 0.5 ug/ml on Recombinat lysates.)
IHC (Immunohistochemistry)
(Figure-2: Immunohistochemical analysis of PD-L1 in Hodgkin's Lymphoma tissue using PD-L1 antibody (Clone: ABM4E54) at 5 ug/ml..)
IHC (Immunohistochemistry)
(Figure-2: Immunohistochemical analysis of PD-L1 in Hodgkin's Lymphoma tissue using PD-L1 antibody (Clone: ABM4E54) at 5 ug/ml..)
Application Data
(Figure-1: Cell Surface FLOW analysis of PD-L1 in PHA treated human PBMC using 1 ug of PD-L1 antibody (Clone: ABM4E54). Green represents isotype control; red represents anti-PD-L1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)
Application Data
(Figure-1: Cell Surface FLOW analysis of PD-L1 in PHA treated human PBMC using 1 ug of PD-L1 antibody (Clone: ABM4E54). Green represents isotype control; red represents anti-PD-L1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)
Related Product Information for anti-PD-L1 antibody
PD-L1 (CD274/B7-H1) is a critical membrane-bound costimulatory molecule belonging to the B7 superfamily that inhibits immune responses through its receptor, PD-1. PD-L1 plays a key role in the pathogenesis of inflammatory diseases (programmed death 1). It is widely expressed in the mononuclear phagocyte system (MPS), may co-stimulate T cells, and regulates inflammatory responses. PD-L1 exerts inflammation regulatory functions via a negative co-stimulatory effect on T cell functions to inhibit cytokine secretion, facilitate apoptosis of activated T cells, and induce T cell anergy. Aberrant expression and dysregulation of CD274 have been reported during bacterial infection, inflammation, and in numerous autoimmune diseases.
Product Categories/Family for anti-PD-L1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,275 Da
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform b
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
Similar Products
Product Notes
The PD-L1 cd274 (Catalog #AAA14889) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Human PD-L1 (Clone: ABM4E54)(BSA/AZIDE Free) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PD-L1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB). WB: 2-4 ug/ml IHC: 5-10 ug/ml FACS: 1-2 ug/ml. Researchers should empirically determine the suitability of the PD-L1 cd274 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-L1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.