Mouse PINK1 Monoclonal Antibody | anti-PINK1 antibody

PINK1 Antibody

Cat. Num. AAA102887

• Gene Names: PINK1; BRPK; PARK6
• Reactivity: Human, Rat, Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot
• Synonyms: PINK1, Antibody; PINK1 Antibody; Parkinson disease (autosomal recessive) 6; EC 2.7.11.1; PARK6; serine/threonine-protein kinase PINK1 mitochondrial; PTEN-induced putative kinase protein 1; protein kinase BRPK; BRPK; FLJ27236; PTEN induced putative kinase 1; Phosphatase and Tensin Homolog; anti-PINK1 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S4-15
• Specificity: Detects ~50kDa
• Form/Format: Protein G Purified; PBS pH 7.4, 50% glycerol, 0.1% sodium azide
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 581

• Applicable Applications for anti-PINK1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
• Certificate of Analysis: 1ug/mL was sufficient for detection of PINK1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Immunogen: Fusion protein amino acids 112-496 (cytoplasmic C-terminus) of human PINK1. 82% identical to rate and 81% identical to mouse. >30% identity with DMPK.
• Research Area(s): Neuroscience, Cell Signaling, Post-translational Modifications, Phosphorylation, Cadiovascular system, Heart.
• Cellular Localization: Mitchondrion|Mitchondrion outer membrane|Cytoplasm.
• Preparation and Storage: Ships on blue ice. -20 degree C; 1yr+ Avoid freeze/thaw cycle.

ICC (Immunocytochemistry)

(1. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15 (AAA102887). Tissue: Neuroblastoma cells. Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody (AAA102887) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) PINK1 Antibody (D) Composite.)

WB (Western Blot)

(Western Blot analysis of Rat Brain showing detection of ~50 kDa PINK1 protein using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa.)

Related Product Information for anti-PINK1 antibody

PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kD protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kD and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports; PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction.

Product Categories/Family for anti-PINK1 antibody

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 14165272
• NCBI GeneID: 65018
• NCBI Accession #: NP_115785.1
• NCBI GenBank Nucleotide #: NM_032409.2
• UniProt Accession #: Q9BXM7.1; Q8N6T9; Q8NBU3; Q96DE4; Q9BXM7
• NCBI Official Full Name: serine/threonine-protein kinase PINK1, mitochondrial
• NCBI Official Synonym Full Names: PTEN induced putative kinase 1
• NCBI Official Symbol: PINK1
• NCBI Official Synonym Symbols: BRPK; PARK6
• NCBI Protein Information: serine/threonine-protein kinase PINK1, mitochondrial; protein kinase BRPK; PTEN-induced putative kinase protein 1
• UniProt Protein Name: Serine/threonine-protein kinase PINK1, mitochondrial
• UniProt Gene Name: PINK1
• UniProt Entry Name: PINK1_HUMAN