Podoplanin Recombinant Antibody | Pdpn recombinant antibody

Anti-Podoplanin (MAP tag) [PMab-1], Goat IgG, kappa

Cat. Num. AAA72497

• Gene Names: Pdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A affinity purified
• Synonyms: Podoplanin, Recombinant Antibody; Anti-Podoplanin (MAP tag) [PMab-1], Goat IgG, kappa; Podoplanin (MAP tag); Anti-Podoplanin (MAP tag) [PMab-1]; PDPN; Aggrus; Glycoprotein 38; Gp38; OTS-8; Ots8; PA2.26 antigen; T1-alpha; T1A; Transmembrane glycoprotein E11; Pdpn recombinant antibody

• Host: Goat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Goat IgG kappa
• Clone Number: PMab-1
• Specificity: This antibody is specific for amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin (PDPN). This antibody, therefore, possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), which is derived from the PLAG domain of murine podoplanin. PDPN is a type I transmembrane protein that interacts with the platelet receptor C-type lectin-like receptor-2 (CLEC-2). PDPN is expressed by a wide variety of cells, including podocytes, lymphatic endothelial cells and type I alveolar cells. PDPN is also upregulated on certain cancer cells, where it mediates tumour-induced platelet aggregation by binding to CLEC-2 on platelets. Chihara (2018) associated Podoplanin with a co-inhibitory receptor in T-cell activation along with PD-1, TIM-3, LAG-3 and TIGIT.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for Pdpn recombinant antibody: Blocking, FCM/FACS (Flow Cytometry), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Chimeric Use Statement: This chimeric goat antibody was made using the variable domain sequences of the original rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

IF (Immunofluorescence)

(ImmunofluorescencestainingofHEK293cellswithAnti-Podoplanin(MAPtag)(AAA72497)PMab-1 ImmunofluorescenceanalysisofparaformaldehydefixedHEK293cellstransfectedwithPodoplanin(MAPtag)expressingplasmid(A)andnon-transfectedHEK293cells(B),permeabilizedwith0.15%TritonstainedwiththechimericrabbitIgGversionofPMab-1(AAA72497)(1:200dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).)

WB (Western Blot)

(Western BlotusingAnti-Podoplanin(MAPtag)antibodyPMab-1(AAA72497). Podoplanin(MAPtag)expressingplasmidtransfected(A)andnon-transfected(B)HEK293cellslysate(3ugproteininRIPAbuffer)wereresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofPMab-1(AAA72497)at0.00001ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)

ELISA

(ELISAusingPMab-1(AAA72497)andMultiTagprotein2. BindingcurvesofthemouseIgG1chimericversionoftheanti-MAPantibodyPMab-1(AAA72497-1.1;edline)andisotypecontroltoanELISAplatecoatedwithMultiTagprotein2(Pr00565,)ataconcentrationof5ug/ml.Ab02007antibodywastitratedona3-foldserialdilutionstartingat10,000ng/mL.Anti-mouseIgGwasusedassecondaryantibodyfordetectionatadilutionof1:4,000.)

FCM/FACS (Flow Cytometry)

(Flow-cytometryusingtheanti-Podoplanin(MAPtag)antibodyPMab-1(AAA72497) NIH3T3cellswerestainedwithanti-FluoresceinIgGantibody(4-4-20;isotypecontrol,blackline)ortherabbitIgG-chimericversionofPMab-1(AAA72497,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)

WB (Western Blot)

(Western Blotusinganti-Podoplanin(MAPtag)antibodyPMab-1(AAA72497) Mousethymus(A),mousebrain(B),NIH3T3cells(C)andmousekidney(D)lysates(35ugproteininRIPAbuffer)wereresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitIgGversionof1B4at0.01ug/ml,0.1ug/ml,0.001ug/mland0.003ug/ml,respectively,beforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)

Related Product Information for Pdpn recombinant antibody

This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.

References

Kaji et al Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin Acta Histochem. Cytochem. 45 (4): 227-237, 2012. doi:10.1267/ahc.12008 N/A23012488
Note on publication:
Describes the original generation and characterisation of this antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 595763348
• NCBI GeneID: 14726
• NCBI Accession #: NP_001277751.1
• NCBI GenBank Nucleotide #: NM_001290822.1
• UniProt Accession #: Q62011; Q546R8; Q61612; A2A8J3
• Molecular Weight: 18,233 Da
• NCBI Official Full Name: podoplanin isoform 2
• NCBI Official Synonym Full Names: podoplanin
• NCBI Official Symbol: Pdpn
• NCBI Official Synonym Symbols: T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
• NCBI Protein Information: podoplanin
• UniProt Protein Name: Podoplanin
• UniProt Gene Name: Pdpn
• UniProt Synonym Gene Names: Gp38; Ots8; Gp38; T1A