FCM/FACS (Flow Cytometry)
(Flow cytometry using the anti-PRMT5 antibody RAB-C136 (AAA72206). HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody or the rabbit IgG1 version of RAB-C136 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Human anti-Human PRMT5 Monoclonal Antibody | anti-PRMT5 antibody
Anti-PRMT5 [RAB-C136]
Gene Names
PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Immunofluorescence, ELISA
Purity
Purified antibody. Purified by Immobilized Metal Affinity Chromatography
Synonyms
PRMT5, Antibody; Anti-PRMT5 [RAB-C136]; PRMT5; Protein arginine N-methyltransferase 5; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog; SKB1 homolog; SKB1Hs; anti-PRMT5-RAB-C136; 48F3_10; anti-PRMT5 antibody
Host
Human
Reactivity
Human
Clonality
Monoclonal
Isotype
Human Fab fragment, kappa
Clone Number
RAB-C136
Specificity
This antibody recognizes PRMT5 (Protein arginine N-methyltransferase 5). It binds to a folded domain, amino acids 269-637. PRMT5 is a arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA.
Purity/Purification
Purified antibody. Purified by Immobilized Metal Affinity Chromatography
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PRMT5 antibody
ChIP (Chromatin immunoprecipitation), IP-MS (Immunoprecipitation-MassSpectrometry), IF (Immunofluorescence), ELISA
Heavy Chain Modification
His-Tagged
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometry using the anti-PRMT5 antibody RAB-C136 (AAA72206). HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody or the rabbit IgG1 version of RAB-C136 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the anti-PRMT5 antibody RAB-C136 (AAA72206). HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody or the rabbit IgG1 version of RAB-C136 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed HeLa cells with anti-PRMT5 antibody RAB-C136 (AAA72206) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RAB-C136 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed HeLa cells with anti-PRMT5 antibody RAB-C136 (AAA72206) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RAB-C136 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-PRMT5 antibody
Recombinant monoclonal antibody to PRMT5. Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody RAB-C136.
This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRMT5 protein under non-denaturing conditions.
This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRMT5 protein under non-denaturing conditions.
Product Categories/Family for anti-PRMT5 antibody
References
N/A
Note on publication: More information on this antibody can be found on the website of the Recombinant Antibody Network.
Note on publication: More information on this antibody can be found on the website of the Recombinant Antibody Network.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
637
NCBI Official Full Name
Protein arginine N-methyltransferase 5
NCBI Official Synonym Full Names
protein arginine methyltransferase 5
NCBI Official Symbol
PRMT5
NCBI Official Synonym Symbols
JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
NCBI Protein Information
protein arginine N-methyltransferase 5; SKB1 homolog; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5
UniProt Protein Name
Protein arginine N-methyltransferase 5
UniProt Gene Name
PRMT5
UniProt Synonym Gene Names
HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
UniProt Entry Name
ANM5_HUMAN
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Product Notes
The PRMT5 prmt5 (Catalog #AAA72206) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRMT5 [RAB-C136] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRMT5 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IP-MS (Immunoprecipitation-MassSpectrometry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the PRMT5 prmt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRMT5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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