Rabbit PRMT5 Polyclonal Antibody | anti-PRMT5 antibody

PRMT5 Polyclonal Antibody

Cat. Num. AAA37161

• Gene Names: PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity Purification
• Synonyms: PRMT5, Antibody; PRMT5 Polyclonal Antibody; PRMT5; Skb1Hs Methyltransferase: HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; Skb1Hs Methyltransferase; anti-PRMT5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 637

• Applicable Applications for anti-PRMT5 antibody: ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Species: Human
• Route: Synthetic Peptide
• Immunogen: A synthetic peptide of human PRMT5
• Calculated Molecular Weight: 73kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cells using PRMT5 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human colon using PRMT5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded mouse brain using PRMT5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded rat brain using PRMT5 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using PRMT5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)

Related Product Information for anti-PRMT5 antibody

Human Skb1Hs methyltransferase (also called JBP1), a homologue of yeast protein Skb1 and Hsl7p (1,2), is composed of 637 amino acid residues and contains motifs conserved among protein methyltransferases. It methylates histones and MBP in vitro (2). Yeast Hsl7p is involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p (3). An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast two-hybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK) (4). Both proteins belong to the protein methyltransferase superfamily (5). Interestingly, human Skb1Hs methyltransferase was shown to interact with Jak kinases. This suggests the possibility that the Skb1Hs methyltransferase could link Jak to a PAK signaling pathway in mammalian cells.

Product Categories/Family for anti-PRMT5 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 32171585
• NCBI GeneID: 10419
• UniProt Accession #: O14744; Q6IBR1; Q9UKH1; A8MTP3; A8MZ91; B4DX49; B4DY30; B5BU10; D3DS33; E2QRE7
• Molecular Weight: 637
• NCBI Official Full Name: Protein arginine N-methyltransferase 5
• NCBI Official Synonym Full Names: protein arginine methyltransferase 5
• NCBI Official Symbol: PRMT5
• NCBI Official Synonym Symbols: JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• NCBI Protein Information: protein arginine N-methyltransferase 5; SKB1 homolog; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5
• UniProt Protein Name: Protein arginine N-methyltransferase 5
• UniProt Gene Name: PRMT5
• UniProt Synonym Gene Names: HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
• UniProt Entry Name: ANM5_HUMAN