SDS-PAGE
(The purity of PRMT5 was determined to be >80% by densitometry, approx. MW 96 kDa.)
PRMT5 recombinant protein
PRMT5 recombinant protein
Gene Names
PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
Applications
Western Blot
Purity
>80%
Synonyms
PRMT5; N/A; PRMT5 recombinant protein; HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs
Host
Sf9 insect cells
Purity/Purification
>80%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1 ug/ul (varies by lot)
Sequence Length
2541
Applicable Applications for PRMT5 recombinant protein
WB (Western Blot), Kinase Assay
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human PRMT5 was expressed by baculovirus in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(The purity of PRMT5 was determined to be >80% by densitometry, approx. MW 96 kDa.)
SDS-PAGE
(The purity of PRMT5 was determined to be >80% by densitometry, approx. MW 96 kDa.)
Related Product Information for PRMT5 recombinant protein
Recombinant full-length human PRMT5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PRMT5 is a protein arginine methyltransferase and part of a 20S methyltransferase complex that modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (1). This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. MEP50 associates with PRMT5 in the 20S arginine methyltransferase complex in HeLa cells (2). Antibodies directed against MEP50 can reduce the arginine methyltransferase activity of the immunopurified complex toward Sm substrates. PRMT5-mediated transcriptional repression of reporter gene is dependent on MEP50.
Scientific Background: PRMT5 is a protein arginine methyltransferase and part of a 20S methyltransferase complex that modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (1). This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. MEP50 associates with PRMT5 in the 20S arginine methyltransferase complex in HeLa cells (2). Antibodies directed against MEP50 can reduce the arginine methyltransferase activity of the immunopurified complex toward Sm substrates. PRMT5-mediated transcriptional repression of reporter gene is dependent on MEP50.
Product Categories/Family for PRMT5 recombinant protein
References
1. Friesen, W. J. et al: A novel WD repeat protein component of the methylosome binds Sm proteins. J. Biol. Chem. 277: 8243-8247, 2002.
2. Furuno, K. et al: Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro. Biochem. Biophys. Res. Commun. 345: 1051-1058, 2006.
2. Furuno, K. et al: Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro. Biochem. Biophys. Res. Commun. 345: 1051-1058, 2006.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~96 kDa
NCBI Official Full Name
Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mRNA
NCBI Official Synonym Full Names
protein arginine methyltransferase 5
NCBI Official Symbol
PRMT5
NCBI Official Synonym Symbols
JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
NCBI Protein Information
protein arginine N-methyltransferase 5; SKB1 homolog; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5
UniProt Protein Name
Protein arginine N-methyltransferase 5
UniProt Gene Name
PRMT5
UniProt Synonym Gene Names
HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
UniProt Entry Name
ANM5_HUMAN
Similar Products
Product Notes
The PRMT5 prmt5 (Catalog #AAA72884) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human PRMT5 was expressed by baculovirus in Sf9 insect cells. AAA Biotech's PRMT5 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the PRMT5 prmt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRMT5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.