WB (Western Blot)
(HMT assay for recombinant PRMT5 protein activity 0.25 ug of Histone H4 was incubated with 0 ug, 0.075 ug, 0.15 ug, and 0.3 ug PRMT5 protein in a 30 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hr at room temperature. Reaction products were run on a 12.5% SDS-PAGE and detected by H4R3me2s antibody . PRMT5 only was used as negative control. Western Blot was used for activity detection.)
PRMT5 recombinant protein
Recombinant PRMT5 protein
Gene Names
PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
Purity
The recombinant protein is >90% pure by SDS-PAGE.
Synonyms
PRMT5; N/A; Recombinant PRMT5 protein; Protein Arginine Methyltransferase 5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5; PRMT5 recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >90% pure by SDS-PAGE.
Form/Format
Recombinant PRMT5 protein is supplied in 25mM HEPES pH7.5, 300mM NaCl,10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant PRMT5 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. PRMT5 is an active H3R8 and H4R3 methyltransferase. HMT Assay Conditions: 0.25ug of Histone H4 was incubated with 0ug, 0.075ug, 0.15ug, and 0.3ug PRMT5 protein in a 30ul reaction system containing 50mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 50uM SAM for 3 hr at room temperature. Reaction products were run on a 12.5% SDS-PAGE and detected by H4R3me2s antibody. PRMT5 only was used as negative control. Western Blot was used for activity detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
WB (Western Blot)
(HMT assay for recombinant PRMT5 protein activity 0.25 ug of Histone H4 was incubated with 0 ug, 0.075 ug, 0.15 ug, and 0.3 ug PRMT5 protein in a 30 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hr at room temperature. Reaction products were run on a 12.5% SDS-PAGE and detected by H4R3me2s antibody . PRMT5 only was used as negative control. Western Blot was used for activity detection.)
WB (Western Blot)
(HMT assay for recombinant PRMT5 protein activity 0.25 ug of Histone H4 was incubated with 0 ug, 0.075 ug, 0.15 ug, and 0.3 ug PRMT5 protein in a 30 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hr at room temperature. Reaction products were run on a 12.5% SDS-PAGE and detected by H4R3me2s antibody . PRMT5 only was used as negative control. Western Blot was used for activity detection.)
SDS-PAGE
(Recombinant PRMT5 protein gel. PRMT5 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 74 kDa Purity: > 90%)
SDS-PAGE
(Recombinant PRMT5 protein gel. PRMT5 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 74 kDa Purity: > 90%)
Related Product Information for PRMT5 recombinant protein
Short Description: Recombinant PRMT5 (accession number NP_006100.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 74.2 kDa. PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10. Recombinant PRMT5 can be used to study enzyme kinetics and perform inhibitor screening assays.
Background: PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.
Background: PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.
Product Categories/Family for PRMT5 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,311 Da
NCBI Official Full Name
protein arginine N-methyltransferase 5 isoform a
NCBI Official Synonym Full Names
protein arginine methyltransferase 5
NCBI Official Symbol
PRMT5
NCBI Official Synonym Symbols
JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
NCBI Protein Information
protein arginine N-methyltransferase 5; SKB1 homolog; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5
UniProt Protein Name
Protein arginine N-methyltransferase 5
UniProt Gene Name
PRMT5
UniProt Synonym Gene Names
HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
UniProt Entry Name
ANM5_HUMAN
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Product Notes
The PRMT5 prmt5 (Catalog #AAA59657) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PRMT5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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