IHC (Immunohiostchemistry)
(Leica Bond-Max; Immunohistochemical staining patterns of formalin fixed and paraffin embedded human prostate tissue (4 um sections) with Anti - PSA monospecific antibody, according to datasheet, using Leica Bond-Max. The prostate gland tissues show a strong distinct cytoplasmic PSA expression.)
Rabbit PSA (Prostate-specific antigen) Monoclonal Antibody | anti-PSA antibody
Anti-PSA (Prostate-specific antigen)
Applications
Immunohistochemistry
Synonyms
PSA (Prostate-specific antigen), Antibody; Anti-PSA (Prostate-specific antigen); anti-PSA antibody
Host
Rabbit
Clonality
Monoclonal
Clone Number
L15-V
Specificity
Human, mouse, rat
Form/Format
20 mM Tris-HCl, pH 8.0; 20 mg/ml BSA; 0.05% NaN3
Applicable Applications for anti-PSA antibody
IHC (Immunohistochemistry)
Immunogen
Peptide derived from N-terminal sequence of human PSA. Antibody recognizes the epitope between Arg77-Gln88
Cellular Localization
Cytoplasm
Positive Control
Prostate hyperplasia
Protein Accession Number
P07288
Ventana Protocol
Procedure: U ultraView DAB
1. Deparafinization
2. Heating (72°C) at the medium temperatures. Deparafinization
3. Cell conditioning
4. ULTRA conditioner #1
5. Heating glass (95°C), incubation 8 min. (Cell conditioner #1; buffer CC1)
6. ULTRA CC1 solution application - 36 min.
7. Antibody incubation temperature
8. Heating glass (36°C), incubation 4 min.
9. Titration
10. Hand apply - primary antibody 100 ul. Incubation 36 min.
11. ultraWash
12. Nuclear stain
13. Hematoxylin II application - one drop (nuclear stain). Cover and incubate 12 min.
14. After nuclear stain
15. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min.
1. Deparafinization
2. Heating (72°C) at the medium temperatures. Deparafinization
3. Cell conditioning
4. ULTRA conditioner #1
5. Heating glass (95°C), incubation 8 min. (Cell conditioner #1; buffer CC1)
6. ULTRA CC1 solution application - 36 min.
7. Antibody incubation temperature
8. Heating glass (36°C), incubation 4 min.
9. Titration
10. Hand apply - primary antibody 100 ul. Incubation 36 min.
11. ultraWash
12. Nuclear stain
13. Hematoxylin II application - one drop (nuclear stain). Cover and incubate 12 min.
14. After nuclear stain
15. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min.
Leica Bond Max Protocol
Protocol F
Visualization system: BOND Refine DS9800
Epitope retrieval/heating time/temperature: ER2/30 min./100°C
Incubation of primary antibody/temperature: 30 min./20°C
Visualization system: BOND Refine DS9800
Epitope retrieval/heating time/temperature: ER2/30 min./100°C
Incubation of primary antibody/temperature: 30 min./20°C
Preparation and Storage
Store at 4°C. Do not freeze.
IHC (Immunohiostchemistry)
(Leica Bond-Max; Immunohistochemical staining patterns of formalin fixed and paraffin embedded human prostate tissue (4 um sections) with Anti - PSA monospecific antibody, according to datasheet, using Leica Bond-Max. The prostate gland tissues show a strong distinct cytoplasmic PSA expression.)
IHC (Immunohiostchemistry)
(Leica Bond-Max; Immunohistochemical staining patterns of formalin fixed and paraffin embedded human prostate tissue (4 um sections) with Anti - PSA monospecific antibody, according to datasheet, using Leica Bond-Max. The prostate gland tissues show a strong distinct cytoplasmic PSA expression.)
IHC (Immunohistochemistry)
(Ventana BenchMark; Immunohistochemical staining patterns of formalin fixed and paraffin embedded human prostate tissue (4 um sections) with Anti - PSA monospecific antibody, according to datasheet, using Ventana BenchMark. The prostate gland tissues show a strong distinct cytoplasmic PSA expression.)
IHC (Immunohistochemistry)
(Ventana BenchMark; Immunohistochemical staining patterns of formalin fixed and paraffin embedded human prostate tissue (4 um sections) with Anti - PSA monospecific antibody, according to datasheet, using Ventana BenchMark. The prostate gland tissues show a strong distinct cytoplasmic PSA expression.)
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The PSA (Catalog #AAA79023) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PSA (Prostate-specific antigen) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PSA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSA (Prostate-specific antigen), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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