Mouse RAD51 Monoclonal Antibody | anti-RAD51 antibody

RAD51 Antibody

Cat. Num. AAA28804

• Gene Names: RAD51; RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Synonyms: RAD51, Antibody; RAD51 Antibody; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A; RAD51; RAD51A; RECA; anti-RAD51 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,kappa
• Specificity: This RAD51 antibody is generated from a mouse immunized with a recombination protein from human.
• Purity/Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 340

• Applicable Applications for anti-RAD51 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Source: Human
• Function: Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Recruited to resolve stalled replication forks during replication stress. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair.
• Cellular Location: Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage (PubMed:20154705). DNA damage induces an increase in nuclear levels (PubMed:20154705). Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment (PubMed:23754376). Accumulated at sites of DNA damage in a SPIDR- dependent manner (PubMed:23509288). Recruited at sites of DNA damage in a MCM9-MCM8-dependent manner (PubMed:23401855). Colocalizes with ERCC5/XPG to nuclear foci in S phase (PubMed:26833090). Recruited to stalled replication forks during replication stress by the TONSL-MMS22L complex, as well as ATAD5 and WDR48 in an ATR-dependent manner (PubMed:27797818, PubMed:31844045).
• Tissue Location: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of lysates from Jurkat, mouse NIH/3T3, rat C6, rat PC-12, Hela cell line (from left to right), using RAD51 AAA28804 Antibody. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Related Product Information for anti-RAD51 antibody

Background: Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

References

Shinohara A.,et al.Nat. Genet. 4:239-243(1993). Yoshimura Y.,et al.Nucleic Acids Res. 21:1665-1665(1993). Schmutte C.,et al.Cancer Res. 59:4564-4569(1999). Wang W.W.,et al.Cancer Epidemiol. Biomarkers Prev. 10:955-960(2001). Park J.Y.,et al.Nucleic Acids Res. 36:3226-3234(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 256017143
• NCBI GeneID: 5888
• NCBI Accession #: NP_001157741.1
• NCBI GenBank Nucleotide #: NP_001157741.1
• UniProt Accession #: Q06609
• Molecular Weight: Calculated MW: H=37,26,31 KDa
• NCBI Official Full Name: DNA repair protein RAD51 homolog 1 isoform 2
• NCBI Official Synonym Full Names: RAD51 recombinase
• NCBI Official Symbol: RAD51
• NCBI Official Synonym Symbols: RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• NCBI Protein Information: DNA repair protein RAD51 homolog 1
• UniProt Protein Name: DNA repair protein RAD51 homolog 1
• UniProt Gene Name: RAD51
• UniProt Synonym Gene Names: RAD51A; RECA; HsRAD51; hRAD51
• UniProt Entry Name: RAD51_HUMAN