ICC (Immunocytochemistry)
(Immunocytochemistry of Raf1 (green) in MCF-7 using Raf1 antibody at dilution 1/20, and DAPI(blue))
anti-Human Raf1 Monoclonal Antibody | anti-Raf1 antibody
Raf1 Rabbit mAb
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf
Reactivity
Human
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purified
Synonyms
Raf1, Antibody; Raf1 Rabbit mAb; NS5; CRAF; Raf-1; c-Raf; CMD1NN; anti-Raf1 antibody
Host
Recombinant Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
S05-9B1
Purity/Purification
Affinity Purified
Form/Format
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration
0.3mg/ml (varies by lot)
Applicable Applications for anti-Raf1 antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthetic peptide of human Raf1
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.
ICC (Immunocytochemistry)
(Immunocytochemistry of Raf1 (green) in MCF-7 using Raf1 antibody at dilution 1/20, and DAPI(blue))
ICC (Immunocytochemistry)
(Immunocytochemistry of Raf1 (green) in MCF-7 using Raf1 antibody at dilution 1/20, and DAPI(blue))
WB (Western Blot)
(Western blot detection of Raf1 in C6,3T3,Hela cell lysates using Raf1 Rabbit mAb(1:1000 diluted).Predicted band size:73kDa.Observed band size:73kDa.)
WB (Western Blot)
(Western blot detection of Raf1 in C6,3T3,Hela cell lysates using Raf1 Rabbit mAb(1:1000 diluted).Predicted band size:73kDa.Observed band size:73kDa.)
Related Product Information for anti-Raf1 antibody
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Product Categories/Family for anti-Raf1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,052 Da
NCBI Official Full Name
RAF proto-oncogene serine/threonine-protein kinase
NCBI Official Synonym Full Names
v-raf-1 murine leukemia viral oncogene homolog 1
NCBI Official Symbol
RAF1
NCBI Official Synonym Symbols
NS5; CRAF; Raf-1; c-Raf
NCBI Protein Information
RAF proto-oncogene serine/threonine-protein kinase; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase
UniProt Protein Name
RAF proto-oncogene serine/threonine-protein kinase
UniProt Gene Name
RAF1
UniProt Synonym Gene Names
RAF; cRaf
UniProt Entry Name
RAF1_HUMAN
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Product Notes
The Raf1 raf1 (Catalog #AAA314449) is an Antibody produced from Recombinant Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Raf1 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Raf1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Raf1 raf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Raf1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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