IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Phospho-Ser259) Antibody.)
Rabbit Raf1 Polyclonal Antibody | anti-Raf1 antibody
Raf1 (Phospho-Ser259) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
Raf1, Antibody; Raf1 (Phospho-Ser259) Antibody; C-RAF; C-Raf; CRAF; anti-Raf1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
648
Applicable Applications for anti-Raf1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine 259 (S-T-S(p)-T-P) derived from Human Raf1.
Modification
Phospho-Ser259
Target Name
Raf1
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Phospho-Ser259) Antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Phospho-Ser259) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293 and MDA cells untreated or treated with PMA using Raf1(Phospho-Ser259) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293 and MDA cells untreated or treated with PMA using Raf1(Phospho-Ser259) Antibody.)
Related Product Information for anti-Raf1 antibody
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
Product Categories/Family for anti-Raf1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,395 Da
NCBI Official Full Name
RAF proto-oncogene serine/threonine-protein kinase
NCBI Official Synonym Full Names
Raf-1 proto-oncogene, serine/threonine kinase
NCBI Official Symbol
RAF1
NCBI Official Synonym Symbols
NS5; CRAF; Raf-1; c-Raf; CMD1NN
NCBI Protein Information
RAF proto-oncogene serine/threonine-protein kinase
UniProt Protein Name
RAF proto-oncogene serine/threonine-protein kinase
UniProt Gene Name
RAF1
UniProt Synonym Gene Names
RAF; cRaf
UniProt Entry Name
RAF1_HUMAN
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Product Notes
The Raf1 raf1 (Catalog #AAA302437) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Raf1 (Phospho-Ser259) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Raf1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Raf1 raf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Raf1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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