IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cell using RAF1 antibody. Blue: DAPI for nuclear staining.)
Rabbit RAF1 Polyclonal Antibody | anti-RAF1 antibody
RAF1 Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Antigen affinity purification
Synonyms
RAF1, Antibody; RAF1 Antibody; RAF1; CRAF; NS5; Raf-1; c-Raf; anti-RAF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
1mg/mL (varies by lot)
Sequence Length
421
Applicable Applications for anti-RAF1 antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen
C term -peptide of human RAF1
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-227 / sc-133 / sc-7198
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cell using RAF1 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cell using RAF1 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of mouse brain and mouse heart tissue, using RAF1 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of mouse brain and mouse heart tissue, using RAF1 antibody.)
Related Product Information for anti-RAF1 antibody
A-Raf, B-Raf and RAF1 (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of RAF1 is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate RAF1 at Ser338 and the Src family phosphorylates Tyr341 to induce RAF1 activity (3, 4). Ser338 of RAF1 corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on RAF1 (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6, 7). While A-Raf, B-Raf and RAF1 are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428 and Thr439) and lacks a site equivalent to Tyr341 of RAF1 (8, 9). The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of RAF1 (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with RAF1 inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders RAF1 unresponsive to subsequent activation events (11).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
75,395 Da
NCBI Official Full Name
RAF1, partial
NCBI Official Synonym Full Names
Raf-1 proto-oncogene, serine/threonine kinase
NCBI Official Symbol
RAF1
NCBI Official Synonym Symbols
NS5; CRAF; Raf-1; c-Raf; CMD1NN
NCBI Protein Information
RAF proto-oncogene serine/threonine-protein kinase; C-Raf proto-oncogene, serine/threonine kinase; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; v-raf-1 murine leukemia viral oncogene homolog 1
UniProt Protein Name
RAF proto-oncogene serine/threonine-protein kinase
UniProt Gene Name
RAF1
UniProt Synonym Gene Names
RAF; cRaf
UniProt Entry Name
RAF1_HUMAN
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Product Notes
The RAF1 raf1 (Catalog #AAA169939) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAF1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAF1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the RAF1 raf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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