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WB (Western Blot)
(Detection of human SMC3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, LNCaP, A-549, MCF-7, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC3 recombinant monoclonal antibody (AAA213575 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
SMC3 recombinant antibody
Rabbit anti-SMC3 Recombinant Monoclonal Antibody [BLR099G]
Gene Names
SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
SMC3, Recombinant Antibody; Rabbit anti-SMC3 Recombinant Monoclonal Antibody [BLR099G]; BAM; CSPG6; structural maintenance of chromosomes protein 3; SMC3L1; SMC-3; SMC protein 3; HCAP; chromosome-associated polypeptide; chondroitin sulfate proteoglycan 6 (bamacan); Chondroitin sulfate proteoglycan 6; CDLS3; BMH; Bamacan; basement membrane-associated chondroitin proteoglycan; Structural maintenance of chromosomes protein 3; structural maintenance of chromosomes 3; SMC3 recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR099G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for SMC3 recombinant antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1167 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SMC3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, LNCaP, A-549, MCF-7, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC3 recombinant monoclonal antibody (AAA213575 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human SMC3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, LNCaP, A-549, MCF-7, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC3 recombinant monoclonal antibody (AAA213575 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse SMC3 by western blot. Samples: Whole cell lysate (50 ug) from C2C12, 70Z/3, BW5147.3, CTLL-2, EL4, RenCa, NIH 3T3, TCMK-1, A20, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC3 recombinant monoclonal antibody (AAA213575 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse SMC3 by western blot. Samples: Whole cell lysate (50 ug) from C2C12, 70Z/3, BW5147.3, CTLL-2, EL4, RenCa, NIH 3T3, TCMK-1, A20, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC3 recombinant monoclonal antibody (AAA213575 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human SMC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMC3 recombinant monoclonal antibody (AAA213575 lot 1) used for IP at 6 ul per reaction. SMC3 was also immunoprecipitated by rabbit anti-SMC3 antibody For blotting immunoprecipitated SMC3, AAA213575 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human SMC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMC3 recombinant monoclonal antibody (AAA213575 lot 1) used for IP at 6 ul per reaction. SMC3 was also immunoprecipitated by rabbit anti-SMC3 antibody For blotting immunoprecipitated SMC3, AAA213575 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
FCM/FACS (Flow Cytometry)
(Detection of human SMC3 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-SMC3 recombinant monoclonal antibody (AAA213575 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human SMC3 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-SMC3 recombinant monoclonal antibody (AAA213575 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for SMC3 recombinant antibody
SMC3 (structural maintenance of chromosomes 3) is a component of the cohesin complex which is critical for precise duplication and segregation of chromosomes. SMC3 heterodimerizes with SMC1 and associates with two non-SMC subunits, Rad21/Scc1 and Scc3, to form the cohesin complex.
Product Categories/Family for SMC3 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
141,542 Da
NCBI Official Full Name
structural maintenance of chromosomes protein 3
NCBI Official Synonym Full Names
structural maintenance of chromosomes 3
NCBI Official Symbol
SMC3
NCBI Official Synonym Symbols
BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
NCBI Protein Information
structural maintenance of chromosomes protein 3
UniProt Protein Name
Structural maintenance of chromosomes protein 3
UniProt Gene Name
SMC3
UniProt Synonym Gene Names
BAM; BMH; CSPG6; SMC3L1; SMC protein 3; SMC-3; Bamacan; hCAP
UniProt Entry Name
SMC3_HUMAN
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Product Notes
The SMC3 smc3 (Catalog #AAA213575) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SMC3 Recombinant Monoclonal Antibody [BLR099G] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMC3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SMC3 smc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMC3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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