FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human TNF-alpha expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human TNF-alpha, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Rabbit anti-Human TNF-alpha/TNFA/TNFSF2 Monoclonal Antibody | anti-TNF antibody
TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody
Gene Names
TNF; DIF; TNFA; TNFSF2; TNF-alpha
Reactivity
Human
Applications
Neutralization Assay, Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
TNF-alpha/TNFA/TNFSF2, Antibody; TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody; tumor necrosis factor; Anti-DIF Antibody; Anti-TNF-alpha Antibody; Anti-TNFA Antibody; Anti-TNFSF2 Antibody; anti-TNF antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R101
Specificity
Human TNF-alpha/TNFA.
No cross-reactivity in ELISA with Mouse TNFa, Rat TNFa
Has cross-reactivity in ELISA with Cynomolgus TNFa, Canine TNFa, Ferret TNFa
No cross-reactivity in ELISA with Mouse TNFa, Rat TNFa
Has cross-reactivity in ELISA with Cynomolgus TNFa, Canine TNFa, Ferret TNFa
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TNF antibody
NTRL (Neutralization Assay), Blocking, FCM/FACS (Flow Cytometry)
Immunogen
Recombinant Human TNF-alpha/TNFA protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human TNF-alpha/TNFA (rHuman TNF-alpha/TNFA) and was produced using recombinant antibody technology.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human TNF-alpha expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human TNF-alpha, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human TNF-alpha expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human TNF-alpha, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Application Data
(The neutralization activity of antibody is measured by its ability to neutralize TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 13.5-54 ng/mL in the presence of 0.25 ng/mL Recombinant Human TNFa and 1 ug/mL actinomycin D.)
Application Data
(The neutralization activity of antibody is measured by its ability to neutralize TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 13.5-54 ng/mL in the presence of 0.25 ng/mL Recombinant Human TNFa and 1 ug/mL actinomycin D.)
Related Product Information for anti-TNF antibody
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
TNF-alpha Protein & Antibody
Product Categories/Family for anti-TNF antibody
References
Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,644 Da
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNF-alpha
NCBI Protein Information
tumor necrosis factor; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_HUMAN
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Product Notes
The TNF tnf (Catalog #AAA255020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TNF-alpha/TNFA/TNFSF2 can be used in a range of immunoassay formats including, but not limited to, NTRL (Neutralization Assay), Blocking, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the TNF tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF-alpha/TNFA/TNFSF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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