Mouse anti-Human TRIM24/TIF1 alpha Monoclonal Antibody | anti-TRIM24 antibody

TRIM24/TIF1 alpha Mouse Monoclonal Antibody [Clone PCRP-TRIM24-1B12]

Cat. Num. AAA216022

• Gene Names: TRIM24; PTC6; TF1A; TIF1; RNF82; TIF1A; hTIF1; TIF1ALPHA
• Reactivity: Human
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: TRIM24/TIF1 alpha, Antibody; TRIM24/TIF1 alpha Mouse Monoclonal Antibody [Clone PCRP-TRIM24-1B12]; E3 ubiquitin protein ligase TRIM24; hTIF1; PTC6; RING finger protein 82; RNF82; TF1A; TIF1 alpha; Transcriptional intermediary factor 1 alpha; Tripartite motif-containing protein 24; anti-TRIM24 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG1
• Clone Number: PCRP-TRIM24-1B12
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-TRIM24 antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant full-length human TRIM24 protein
• Cellular Localization: Nucleus, Cytoplasm, Colocalizes with sites of active transcription
• Positive Control: HeLa or K562 cells.
• Hu-chromosome Location: 7q33
• Unigene: 490287
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. TRIM24/TIF1a Mouse Monoclonal Antibody (PCRP-TRIM24-1B12) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).)

Related Product Information for anti-TRIM24 antibody

TIF1alpha, also known as TRIM24, mediates transcriptional events by interactions with the AF2 region of several nuclear receptors, such as the estrogen, retinoic acid and vitamin D3 receptors. TIF1alpha localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. TIF1alpha is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains (RING, B-box type 1 and B-box type 2) and a coiled-coil region. The TIF1alpha gene, which maps to human chromosome 7q33, encodes two alternatively spliced transcripts. However, the full length nature of one variant has not been determined. A TIF1alpha homolog (designated bonus) has been identified in Drosophila and is associated with several genes that are implicated in the ecdysone pathway, a nuclear hormone receptor pathway required throughout Drosophila development, suggesting a conserved functional role for the protein throughout the course of evolution.

NCBI and Uniprot Product Information

• NCBI GI #: 47419909
• NCBI GeneID: 8805
• NCBI Accession #: NP_003843.3
• NCBI GenBank Nucleotide #: NM_003852.3
• UniProt Accession #: O15164; O95854; A4D1R7; A4D1R8
• Molecular Weight: 116,831 Da
• NCBI Official Full Name: transcription intermediary factor 1-alpha isoform b
• NCBI Official Synonym Full Names: tripartite motif containing 24
• NCBI Official Symbol: TRIM24
• NCBI Official Synonym Symbols: PTC6; TF1A; TIF1; RNF82; TIF1A; hTIF1; TIF1ALPHA
• NCBI Protein Information: transcription intermediary factor 1-alpha; TIF1-alpha; RING finger protein 82; tripartite motif-containing 24; E3 ubiquitin-protein ligase TRIM24; transcriptional intermediary factor 1
• UniProt Protein Name: Transcription intermediary factor 1-alpha
• UniProt Gene Name: TRIM24
• UniProt Synonym Gene Names: RNF82; TIF1; TIF1A; TIF1-alpha
• UniProt Entry Name: TIF1A_HUMAN