WB (Western Blot)
(Western blot analysis of lysates from mouse NIH/3T3 cell line, mouse spleen, mouse testis, mouse thymus tissue lysate (from left to right), using Trim24 Antibody (C-term). AAA287272 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Rabbit anti-Mouse Trim24 Polyclonal Antibody | anti-Trim24 antibody
(Mouse) Trim24 Antibody (C-term)
Gene Names
Trim24; TIF1; Tif1a; AI447469; TIF1alpha; TIF1-alpha; A130082H20Rik; D430004I05Rik
Reactivity
Mouse
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Trim24, Antibody; (Mouse) Trim24 Antibody (C-term); Transcription intermediary factor 1-alpha; TIF1-alpha; 632-; E3 ubiquitin-protein ligase Trim24; Tripartite motif-containing protein 24; Trim24; Tif1; Tif1a; anti-Trim24 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Trim24 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 789-843 amino acids from the C-terminal region of Mouse Trim24.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
1,017
Applicable Applications for anti-Trim24 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from mouse NIH/3T3 cell line, mouse spleen, mouse testis, mouse thymus tissue lysate (from left to right), using Trim24 Antibody (C-term). AAA287272 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from mouse NIH/3T3 cell line, mouse spleen, mouse testis, mouse thymus tissue lysate (from left to right), using Trim24 Antibody (C-term). AAA287272 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with (Mouse) Trim24 Antibody (C-term). AAA287272 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with (Mouse) Trim24 Antibody (C-term). AAA287272 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
WB (Western Blot)
(Anti-Trim24 Antibody (C-term)at 1:2000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 117 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-Trim24 Antibody (C-term)at 1:2000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 117 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Trim24 antibody
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac) (By similarity). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis via its effects on p53/TP53 levels. Up-regulates ligand- dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, such as RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. Required for normal transition from proliferating neonatal hepatocytes to quiescent adult hepatocytes.
Product Categories/Family for anti-Trim24 antibody
References
le Douarin B.,et al.EMBO J. 14:2020-2033(1995).
le Douarin B.,et al.EMBO J. 15:6701-6715(1996).
Zhong S.,et al.Nat. Genet. 23:287-295(1999).
Seeler J.-S.,et al.Mol. Cell. Biol. 21:3314-3324(2001).
Torres-Padilla M.E.,et al.J. Cell Biol. 174:329-338(2006).
le Douarin B.,et al.EMBO J. 15:6701-6715(1996).
Zhong S.,et al.Nat. Genet. 23:287-295(1999).
Seeler J.-S.,et al.Mol. Cell. Biol. 21:3314-3324(2001).
Torres-Padilla M.E.,et al.J. Cell Biol. 174:329-338(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
116657
NCBI Official Full Name
transcription intermediary factor 1-alpha isoform 2
NCBI Official Synonym Full Names
tripartite motif-containing 24
NCBI Official Symbol
Trim24
NCBI Official Synonym Symbols
TIF1; Tif1a; AI447469; TIF1alpha; TIF1-alpha; A130082H20Rik; D430004I05Rik
NCBI Protein Information
transcription intermediary factor 1-alpha
UniProt Protein Name
Transcription intermediary factor 1-alpha
UniProt Gene Name
Trim24
UniProt Synonym Gene Names
Tif1; Tif1a; TIF1-alpha
UniProt Entry Name
TIF1A_MOUSE
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Product Notes
The Trim24 trim24 (Catalog #AAA287272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Trim24 Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Trim24 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Trim24 trim24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Trim24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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