WB (Western Blot)
(TRIM24 Antibody (N-term)(AAA285572) western blot analysis in U-937,mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the TRIM24 antibody detected the TRIM24 protein (arrow).)
Rabbit anti-Human, mouse TRIM24 Polyclonal Antibody | anti-TRIM24 antibody
TRIM24 Antibody (N-term)
Gene Names
TRIM24; PTC6; TF1A; TIF1; RNF82; TIF1A; hTIF1; TIF1ALPHA
Reactivity
Human, mouse
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TRIM24, Antibody; TRIM24 Antibody (N-term); Transcription intermediary factor 1-alpha; TIF1-alpha; 632-; E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; Tripartite motif-containing protein 24; TRIM24; RNF82; TIF1; TIF1A; anti-TRIM24 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TRIM24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human TRIM24.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
16-45
Applicable Applications for anti-TRIM24 antibody
ELISA, WB (Western Blot)
Function
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity).
Cellular Location
Nucleus. Cytoplasm. Note=Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(TRIM24 Antibody (N-term)(AAA285572) western blot analysis in U-937,mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the TRIM24 antibody detected the TRIM24 protein (arrow).)
WB (Western Blot)
(TRIM24 Antibody (N-term)(AAA285572) western blot analysis in U-937,mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the TRIM24 antibody detected the TRIM24 protein (arrow).)
Related Product Information for anti-TRIM24 antibody
The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Product Categories/Family for anti-TRIM24 antibody
References
Kikuchi, M., et al. Biochim. Biophys. Acta 1793(12):1828-1836(2009)
Lowe, J.K., et al. PLoS Genet. 5 (2), E1000365 (2009) :
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Yondola, M.A., et al. J. Virol. 81(8):4264-4271(2007)
Lowe, J.K., et al. PLoS Genet. 5 (2), E1000365 (2009) :
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Yondola, M.A., et al. J. Virol. 81(8):4264-4271(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
116831
NCBI Official Full Name
transcription intermediary factor 1-alpha isoform b
NCBI Official Synonym Full Names
tripartite motif containing 24
NCBI Official Symbol
TRIM24
NCBI Official Synonym Symbols
PTC6; TF1A; TIF1; RNF82; TIF1A; hTIF1; TIF1ALPHA
NCBI Protein Information
transcription intermediary factor 1-alpha
UniProt Protein Name
Transcription intermediary factor 1-alpha
UniProt Gene Name
TRIM24
UniProt Synonym Gene Names
RNF82; TIF1; TIF1A; TIF1-alpha
UniProt Entry Name
TIF1A_HUMAN
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Product Notes
The TRIM24 trim24 (Catalog #AAA285572) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 16-45. The TRIM24 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM24 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TRIM24 trim24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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