Rabbit UCHL1 Monoclonal Antibody | anti-UCHL1 antibody

Recombinant Anti-UCHL1 Antibody, Rabbit Monoclonal

Cat. Num. AAA255455

• Gene Names: UCHL1; PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, ELISA, Western Blot
• Synonyms: UCHL1, Antibody; Recombinant Anti-UCHL1 Antibody, Rabbit Monoclonal; UCHL1/PGP9.5 Antibody, Rabbit MAb; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Anti-HEL-117 Antibody; Anti-NDGOA Antibody; Anti-PARK5 Antibody; Anti-PGP9.5 Antibody; Anti-PGP95 Antibody; Anti-Uch-L1 Antibody; anti-UCHL1 antibody

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 104
• Specificity: Human UCHL1/PGP9.5
• Form/Format: 0.2um filtered solution in PBS

• Applicable Applications for anti-UCHL1 antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human UCHL1 protein ( )
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human UCHL1 (rh UCHL1).
• Preparation and Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence staining of UCHL1 in DU145 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human UCHL1 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)

IP (Immunoprecipitation)

(UCHL1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg SH-SY5Y Whole Cell LysateLane C:0.5 mg U87MG Whole Cell Lysate2 uL anti-UCHL1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-UCHL1 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size: 25 kDa)

WB (Western Blot)

(Anti-UCHL1 rabbit monoclonal antibody at 1:500 dilutionLane A: SH-SY5Y Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:24 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-UCHL1 antibody

Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.

References

Wada H., et al., 1998, Biochem. Biophys. Res. Commun. 251:688-92. Choi J., et al., 2004, J. Biol. Chem. 279:13256-64. Lombardino, A.2005, et al., J.Proc Natl Acad Sci.USA102 (22):8036-41 Okochi-Takada, E. et al., 2006, Int J Cancer. 119 (6):1338-44. Zetterberg, M. et al., 2010, Mol Neurodegener 5 :11.

NCBI and Uniprot Product Information

• NCBI GI #: 21361091
• NCBI GeneID: 7345
• NCBI Accession #: NP_004172.2
• NCBI GenBank Nucleotide #: NM_004181.4
• UniProt Accession #: P09936; Q4W5K6; Q71UM0
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Gene Name: UCHL1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_HUMAN