<
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with USP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human USP22 Monoclonal Antibody | anti-USP22 antibody
USP22 Antibody
Gene Names
USP22; USP3L
Reactivity
Human
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
USP22, Antibody; USP22 Antibody; Deubiquitinating enzyme 22 antibody; KIAA1063 antibody; Ubiquitin carboxyl terminal hydrolase 22 antibody; Ubiquitin carboxyl-terminal hydrolase 22 antibody; Ubiquitin specific peptidase 22 antibody; Ubiquitin specific peptidase 3 like antibody; Ubiquitin specific processing protease 22 antibody; Ubiquitin specific protease 22 antibody; Ubiquitin thioesterase 22 antibody; Ubiquitin thiolesterase 22 antibody; Ubiquitin-specific-processing protease 22 antibody; UBP22_HUMAN antibody; USP 22 antibody; Usp22 antibody; USP3L antibody; anti-USP22 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JU63-27
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-USP22 antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with USP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with USP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining USP22 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining USP22 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining USP22 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining USP22 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining USP22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining USP22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot)
(Western blot analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution.)
Related Product Information for anti-USP22 antibody
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP22 (ubiquitin specific peptidase 22), also known as USP3L, is a 525 amino acid protein that contains one UBP-type zinc finger and functions to catalyze the conversion of a ubiquitin C-terminal thioester to free ubiquitin and thiol, a reaction that may influence several cellular processes. Via its catalytic activity, USP22 is thought to play an important role in cell cycle progression and may also serve as a cancer stem cell marker. The gene encoding USP22 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1, 200 genes.
Product Categories/Family for anti-USP22 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
525
NCBI Official Full Name
Ubiquitin carboxyl-terminal hydrolase 22
NCBI Official Synonym Full Names
ubiquitin specific peptidase 22
NCBI Official Symbol
USP22
NCBI Official Synonym Symbols
USP3L
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 22; ubiquitin thioesterase 22; deubiquitinating enzyme 22; ubiquitin thiolesterase 22; ubiquitin specific protease 22; ubiquitin-specific processing protease 22; ubiquitin-specific-processing protease 22
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 22
UniProt Gene Name
USP22
UniProt Synonym Gene Names
KIAA1063; USP3L
UniProt Entry Name
UBP22_HUMAN
Similar Products
Product Notes
The USP22 usp22 (Catalog #AAA312563) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP22 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP22 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the USP22 usp22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP22, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.