FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA278960 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
USP22 recombinant antibody
USP22 Recombinant Monoclonal Antibody
Gene Names
USP22; USP3L
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, ELISA
Purity
Affinity purified
Synonyms
USP22, Recombinant Antibody; USP22 Recombinant Monoclonal Antibody; Ubiquitin carboxyl-terminal hydrolase 22 (EC 3.4.19.12)(Deubiquitinating enzyme 22)(Ubiquitin thioesterase 22)(Ubiquitin-specific-processing protease 22); USP22; KIAA1063 USP3L; USP22 recombinant antibody
Host
HEK293F Cell
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
18H6
Purity/Purification
Affinity purified
Form/Format
Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.
Applicable Applications for USP22 recombinant antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from Human USP22
Conjugation
Non-conjugated
Research Area
Epigenetics and Nuclear Signaling; Cell biology
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA278960 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA278960 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA278960 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 507-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA278960 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 507-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
WB (Western Blot)
(Western BlotPositive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysateAll lanes: USP22 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60, 59 kDaObserved band size: 55-72 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysateAll lanes: USP22 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60, 59 kDaObserved band size: 55-72 kDa)
Related Product Information for USP22 recombinant antibody
Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression. {ECO:0000269|PubMed:18206972, ECO:0000269|PubMed:18206973, ECO:0000269|PubMed:18469533}.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
525
NCBI Official Full Name
Ubiquitin carboxyl-terminal hydrolase 22
NCBI Official Synonym Full Names
ubiquitin specific peptidase 22
NCBI Official Symbol
USP22
NCBI Official Synonym Symbols
USP3L
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 22; ubiquitin thioesterase 22; deubiquitinating enzyme 22; ubiquitin thiolesterase 22; ubiquitin specific protease 22; ubiquitin-specific processing protease 22; ubiquitin-specific-processing protease 22
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 22
UniProt Gene Name
USP22
UniProt Synonym Gene Names
KIAA1063; USP3L
UniProt Entry Name
UBP22_HUMAN
Similar Products
Product Notes
The USP22 usp22 (Catalog #AAA278960) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The USP22 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP22 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the USP22 usp22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP22, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.