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IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: Rat Liver Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Mouse anti-Rat Von Hippel Lindau Tumor Suppressor (vHL) Monoclonal Antibody | anti-vHL antibody
Monoclonal Antibody to Von Hippel Lindau Tumor Suppressor (vHL)
Gene Names
Vhl; Vhlh
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Von Hippel Lindau Tumor Suppressor (vHL), Antibody; Monoclonal Antibody to Von Hippel Lindau Tumor Suppressor (vHL); Von Hippel Lindau Tumor Suppressor; HRCA1; RCA1; VHL1; Von Hippel-Lindau Syndrome; anti-vHL antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
C1
Specificity
The antibody is a mouse monoclonal antibody raised against vHL. It has been selected for its ability to recognize vHL in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence Positions
Ala6~Gln175
Applicable Applications for anti-vHL antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL, 1: 500-5000
IHC: 5-20ug/mL, 1: 50-200
ICC: 5-20ug/mL, 1: 50-200
IHC: 5-20ug/mL, 1: 50-200
ICC: 5-20ug/mL, 1: 50-200
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: Rat Liver Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: Rat Liver Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Testis Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Testis Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Spinal cord Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Spinal cord Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: Rat Colon Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: Rat Colon Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Small intestine Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Small intestine Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Cardiac Muscle Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Cardiac Muscle Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Stomach Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Stomach Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Pancreas Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Rat Pancreas Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Sample: Rat Uterus Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Sample: Rat Uterus Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat vHL Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Product Categories/Family for anti-vHL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,215 Da
NCBI Official Full Name
von Hippel-Lindau disease tumor suppressor
NCBI Official Synonym Full Names
von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
NCBI Official Symbol
Vhl
NCBI Official Synonym Symbols
Vhlh
NCBI Protein Information
von Hippel-Lindau disease tumor suppressor
UniProt Protein Name
Von Hippel-Lindau disease tumor suppressor
UniProt Gene Name
Vhl
UniProt Entry Name
VHL_RAT
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Product Notes
The vHL vhl (Catalog #AAA21259) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ala6~Gln175. The Monoclonal Antibody to Von Hippel Lindau Tumor Suppressor (vHL) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Von Hippel Lindau Tumor Suppressor (vHL) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL, 1: 500-5000 IHC: 5-20ug/mL, 1: 50-200 ICC: 5-20ug/mL, 1: 50-200. Researchers should empirically determine the suitability of the vHL vhl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Von Hippel Lindau Tumor Suppressor (vHL), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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