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Mouse anti-Human YAP / YAP1 Monoclonal Antibody | anti-YAP antibody

Mouse Monoclonal [clone 2F12] (IgG2a,k) to Human YAP / YAP1

Gene Names
YAP1; YAP; YKI; COB1; YAP2; YAP65
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
YAP / YAP1, Antibody; Mouse Monoclonal [clone 2F12] (IgG2a,k) to Human YAP / YAP1; Anti-YAP / YAP1 Antibody (clone 2F12) IHC-plus; YAP1; 65 kDa Yes-associated protein; YAP; Yes-associated protein beta; Yes-associated protein delta; Yorkie homolog; YAP2; Yes-associated protein 1; Yes-associated protein 2; YKI; YAP65; Human YAP; anti-YAP antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2F12
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.4.
Concentration
1 mg/ml (varies by lot)
Sequence Length
454
Applicable Applications for anti-YAP antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1 - 5 ug/ml)
Usage: Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (30 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
Target Species
Human
Immunogen Description
YAP1 (NP_006097, 53 a.a. ~ 162 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
YAP / YAP1 antibody was raised against yAP1 (NP_006097, 53 a.a. ~ 162 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

ELISA

(Detection limit for recombinant GST tagged YAP1 is approximately 0.03 ng/ml as a capture antibody.)

WB (Western Blot)

(YAP1 monoclonal antibody clone 2F12 Western blot of YAP1 expression in HeLa NE.)

WB (Western Blot)

(Western blot of YAP1 expression in transfected 293T cell line by YAP1 monoclonal antibody clone 2F12.)

IF (Immunofluorescence)

(Immunofluorescence of monoclonal antibody to YAP1 on HeLa cell (antibody concentration 30 ug/ml).)

IHC (Immunohistochemistry)

(Anti-YAP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry)

(Anti-YAP1 antibody IHC of human colon, muscularis mucosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry)

(Anti-YAP1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,942 Da
NCBI Official Full Name
yorkie homolog isoform 2
NCBI Official Synonym Full Names
Yes-associated protein 1
NCBI Official Symbol
YAP1
NCBI Official Synonym Symbols
YAP; YKI; COB1; YAP2; YAP65
NCBI Protein Information
transcriptional coactivator YAP1; yorkie homolog; protein yorkie homolog; yes-associated protein 2; 65 kDa Yes-associated protein; yes-associated protein YAP65 homolog
UniProt Protein Name
Transcriptional coactivator YAP1
UniProt Gene Name
YAP1
UniProt Synonym Gene Names
YAP65; Yes-associated protein 1
UniProt Entry Name
YAP1_HUMAN

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Product Notes

The YAP yap1 (Catalog #AAA12355) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 2F12] (IgG2a,k) to Human YAP / YAP1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's YAP / YAP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1 - 5 ug/ml) Usage: Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (30 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein). Researchers should empirically determine the suitability of the YAP yap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YAP / YAP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.