Standard Curve (Sample)
Mouse Heat Shock Protein 60 (Hsp60) CLIA Kit | Hsp60 clia kit
Heat Shock Protein 60 (Hsp60) CLIA Kit
Gene Names
Hspd1; 60kDa; CPN60; Hsp60; HSP-60; HSP-65
Reactivity
Mouse
Applications
CLIA (Chemiluminescence Immunoassay)
Synonyms
Heat Shock Protein 60 (Hsp60); N/A; Heat Shock Protein 60 (Hsp60) CLIA Kit; HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1; Chaperonin; Spastic Paraplegia 13; Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein; Hsp60 clia kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Hsp60. No significant cross-reactivity or interference between Hsp60 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.14-100ng/mL
Sensitivity
< 0.04ng/mL
Applicable Applications for Hsp60 clia kit
CLIA (Chemiluminescence Immunoassay)
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hsp60 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hsp60 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Hsp60 clia kit
Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of Hsp60 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Hsp60. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hsp60. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hsp60 level in the sample or standard.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Hsp60. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hsp60. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hsp60 level in the sample or standard.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,015 Da
NCBI Official Full Name
60 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock protein 1 (chaperonin)
NCBI Official Symbol
Hspd1
NCBI Official Synonym Symbols
60kDa; CPN60; Hsp60; HSP-60; HSP-65
NCBI Protein Information
60 kDa heat shock protein, mitochondrial
UniProt Protein Name
60 kDa heat shock protein, mitochondrial
UniProt Gene Name
Hspd1
UniProt Synonym Gene Names
Hsp60; CPN60; HSP-60; Hsp60
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Product Notes
The Mouse Hsp60 hspd1 (Catalog #AAA189320) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA189320 CLIA Kit recognizes Mouse Hsp60. AAA Biotech's Heat Shock Protein 60 (Hsp60) can be used in a range of immunoassay formats including, but not limited to, CLIA (Chemiluminescence Immunoassay). Researchers should empirically determine the suitability of the Hsp60 hspd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 60 (Hsp60), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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