Hsp60 Polyclonal Antibody | anti-Hsp60 antibody

Anti-Hsp60 Antibody

Cat. Num. AAA46392

• Gene Names: HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: Hsp60, Antibody; Anti-Hsp60 Antibody; 60 kDa heat shock protein; 60 kDa chaperonin; 60 kDa heat shock protein, mitochondrial; 60kDa; cb863; CH60_HUMAN; Chaperonin 60; Chaperonin; Chaperonin, 60-KD; CPN60; GROEL; GroEL, E. coli, homolog of; Heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin); Heat shock 60kDa protein 1; Heat shock protein 1 (chaperonin); Heat shock protein 60; Heat shock protein 65; HLD4; Hsp 60; HSP 65; HSP-60; HSP60; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; Short heat shock protein 60 Hsp60s1; Spastic paraplegia 13 (autosomal dominant); SPG13; anti-Hsp60 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 573

• Applicable Applications for anti-Hsp60 antibody: ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived human Hsp60 recombinant protein (Position: A260-Q496). Human Hsp60 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp60.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

ICC (Immunocytochemistry)

(Anti- Hsp60 Picoband antibody, AAA46392, ICCICC: SMMC-7721 Cell)

IHC (Immunohistochemistry)

(Anti- Hsp60 Picoband antibody, AAA46392, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

IHC (Immunohistochemisry)

(Anti- Hsp60 Picoband antibody, AAA46392, IHC(P)IHC(P): Rat Intestine Tissue)

IHC (Immunohiostchemistry)

(Anti- Hsp60 Picoband antibody, AAA46392, IHC(P)IHC(P): Mouse Intestine Tissue)

WB (Western Blot)

(Anti- Hsp60 Picoband antibody, AAA46392, Western blottingAll lanes: Anti Hsp60 (AAA46392) at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: Mouse Kidney Tissue Lysate at 50ugLane 3: HEPA Whole Cell Lysate at 40ugLane 4: NRK Whole Cell Lysate at 40ugLane 5: PC-12 Whole Cell Lysate at 40ugLane 6: SW620 Whole Cell Lysate at 40ugLane 7: A549 Whole Cell Lysate at 40ugLane 8: A431 Whole Cell Lysate at 40ugLane 9: HELA Whole Cell Lysate at 40ugPredicted bind size: 60KDObserved bind size: 60KD)

Related Product Information for anti-Hsp60 antibody

Description: Rabbit IgG polyclonal antibody for 60 kDa heat shock protein, mitochondrial(HSPD1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Background: HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.

References

1. Cheng, M. Y.; Hartl, F.-U.; Martin, J.; Pollock, R. A.; Kalousek, F.; Neupert, W.; Hallberg, E. M.; Hallberg, R. L.; Horwich, A. L. : Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337: 620-625, 1989. 2. Zanin-Zhorov, A.; Cahalon, L.; Tal, G.; Margalit, R.; Lider, O.; Cohen, I. R. : Heat shock protein 60 enhances CD4+CD25+ regulatory T cell function via innate TLR2 signaling. J. Clin. Invest. 116: 2022-2032, 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 31542947
• NCBI GeneID: 3329
• NCBI Accession #: NP_002147.2
• NCBI GenBank Nucleotide #: NM_002156.4
• UniProt Accession #: P10809; Q38L19; Q9UCR6; B2R5M6; B7Z712
• Molecular Weight: 17,100 Da
• NCBI Official Full Name: 60 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock protein family D (Hsp60) member 1
• NCBI Official Symbol: HSPD1
• NCBI Official Synonym Symbols: HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• NCBI Protein Information: 60 kDa heat shock protein, mitochondrial
• UniProt Protein Name: 60 kDa heat shock protein, mitochondrial
• UniProt Gene Name: HSPD1
• UniProt Synonym Gene Names: HSP60; CPN60; HSP-60; Hsp60
• UniProt Entry Name: CH60_HUMAN