WB (Western Blot)
(Detection of human HSP60 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HSP60 antibody AAA212043 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. HSP60 was also immunoprecipitated by rabbit anti-HSP60 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
Rabbit anti-Human HSP60 Polyclonal Antibody | anti-HSP60 antibody
Rabbit anti-HSP60 Antibody, Affinity Purified
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
HSP60, Antibody; Rabbit anti-HSP60 Antibody, Affinity Purified; HLD4; 60 kDa heat shock protein, mitochondrial; P60 lymphocyte protein; mitochondrial matrix protein P1; HuCHA60; HSP65; HSP-60; HSP60; short heat shock protein 60 Hsp60s1; heat shock protein 65; Heat shock protein 60; heat shock 60kDa protein 1 (chaperonin); GROEL; epididymis secretory sperm binding protein; chaperonin 60; SPG13; 60 kDa chaperonin; CPN60; heat shock protein family D (Hsp60) member 1; anti-HSP60 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-HSP60 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine, Orangutan
Immunogen
Between 325 and 375
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human HSP60 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HSP60 antibody AAA212043 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. HSP60 was also immunoprecipitated by rabbit anti-HSP60 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
WB (Western Blot)
(Detection of human HSP60 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HSP60 antibody AAA212043 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. HSP60 was also immunoprecipitated by rabbit anti-HSP60 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
IHC (Immunohistochemistry)
(Detection of human HSP60 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSP60 (Cat. No. AAA212043 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate)
IHC (Immunohistochemistry)
(Detection of human HSP60 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSP60 (Cat. No. AAA212043 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate)
Related Product Information for anti-HSP60 antibody
Heat shock protein 60 (HSP60) is a member of the chaperonin family. HSP60 is a mitochondrial protein that may function as a signaling molecule in the innate immune system. It is essential for the folding and assembly of newly imported proteins in the mitochondria. [taken from NCBI Entrez Gene (GeneID: 3329)].
Product Categories/Family for anti-HSP60 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,055 Da
NCBI Official Full Name
60 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock 60kDa protein 1 (chaperonin)
NCBI Official Symbol
HSPD1
NCBI Official Synonym Symbols
HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
NCBI Protein Information
60 kDa heat shock protein, mitochondrial; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60 lymphocyte protein; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1
UniProt Protein Name
60 kDa heat shock protein, mitochondrial
UniProt Gene Name
HSPD1
UniProt Synonym Gene Names
HSP60; CPN60; HSP-60; Hsp60
UniProt Entry Name
CH60_HUMAN
Similar Products
Product Notes
The HSP60 hspd1 (Catalog #AAA212043) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HSP60 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSP60 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HSP60 hspd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP60, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.