Standard Curve (Sample)
Mouse Integral membrane protein 2B (ITM2B) ELISA Kit | ITM2B elisa kit
Mouse Integral membrane protein 2B (ITM2B) ELISA Kit
Gene Names
ITM2B; BRI; FBD; ABRI; BRI2; E25B; E3-16; imBRI2; BRICD2B
Reactivity
Mouse
Synonyms
Integral membrane protein 2B (ITM2B); N/A; Mouse Integral membrane protein 2B (ITM2B) ELISA Kit; ITM2B elisa kit
Reactivity
Mouse
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ITM2B elisa kit
Intended Uses: This ITM2B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ITM2B. This ELISA kit for research use only!
Principle of the Assay: ITM2B ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ITM2B antibody and an ITM2B-HRP conjugate. The assay sample and buffer are incubated together with ITM2B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITM2B concentration since ITM2B from samples and ITM2B-HRP conjugate compete for the anti-ITM2B antibody binding site. Since the number of sites is limited, as more sites are occupied by ITM2B from the sample, fewer sites are left to bind ITM2B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITM2B concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ITM2B ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ITM2B antibody and an ITM2B-HRP conjugate. The assay sample and buffer are incubated together with ITM2B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITM2B concentration since ITM2B from samples and ITM2B-HRP conjugate compete for the anti-ITM2B antibody binding site. Since the number of sites is limited, as more sites are occupied by ITM2B from the sample, fewer sites are left to bind ITM2B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITM2B concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ITM2B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,283 Da
NCBI Official Full Name
integral membrane protein 2B
NCBI Official Synonym Full Names
integral membrane protein 2B
NCBI Official Symbol
ITM2B
NCBI Official Synonym Symbols
BRI; FBD; ABRI; BRI2; E25B; E3-16; imBRI2; BRICD2B
NCBI Protein Information
integral membrane protein 2B; immature BRI2; ABri/ADan amyloid peptide; transmembrane protein BRI; BRICHOS domain containing 2B
UniProt Protein Name
Integral membrane protein 2B
UniProt Gene Name
ITM2B
UniProt Synonym Gene Names
BRI; BRI2; imBRI2; Bri; mBRI2; BRI2 ICD; Bri2-23
UniProt Entry Name
ITM2B_HUMAN
Similar Products
Product Notes
The Mouse ITM2B itm2b (Catalog #AAA244703) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA244703 ELISA Kit recognizes Mouse ITM2B. It is sometimes possible for the material contained within the vial of "Integral membrane protein 2B (ITM2B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.