Mouse Ubiquitin thioesterase OTU1 (YOD1) ELISA Kit | YOD1 elisa kit

Mouse Ubiquitin thioesterase OTU1 (YOD1) ELISA Kit

Cat. Num. AAA246908

• Gene Names: YOD1; DUBA8; OTUD2; PRO0907
• Reactivity: Mouse
• Synonyms: Ubiquitin thioesterase OTU1 (YOD1); N/A; Mouse Ubiquitin thioesterase OTU1 (YOD1) ELISA Kit; YOD1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of YOD1. No significant cross-reactivity or interference between YOD1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between YOD1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for YOD1 elisa kit

Principle of the Assay: YOD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-YOD1 antibody and an YOD1-HRP conjugate. The assay sample and buffer are incubated together with YOD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YOD1 concentration since YOD1 from samples and YOD1-HRP conjugate compete for the anti-YOD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by YOD1 from the sample, fewer sites are left to bind YOD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YOD1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This YOD1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse YOD1. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for YOD1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 62751964
• NCBI GeneID: 55432
• NCBI Accession #: NP_061036.3
• NCBI GenBank Nucleotide #: NM_018566.3
• Molecular Weight: 33,979 Da
• NCBI Official Full Name: ubiquitin thioesterase OTU1 isoform 1
• NCBI Official Synonym Full Names: YOD1 deubiquitinase
• NCBI Official Symbol: YOD1
• NCBI Official Synonym Symbols: DUBA8; OTUD2; PRO0907
• NCBI Protein Information: ubiquitin thioesterase OTU1; HIN-7; DUBA-8; hsHIN7; OTU domain containing 2; HIV-1-induced protease 7; OTU domain-containing protein 2; YOD1 OTU deubiquinating enzyme 1 homolog ( yeast)
• UniProt Protein Name: Ubiquitin thioesterase OTU1
• UniProt Gene Name: YOD1
• UniProt Synonym Gene Names: DUBA8; HIN7; OTUD2; PRO0907; HIN-7; HsHIN7
• UniProt Entry Name: OTU1_HUMAN