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product-image-AAA283648_IF11.jpg IF (Immunofluorescence) (Confocal immunofluorescent analysis of YOD1 Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Rabbit YOD1 Polyclonal Antibody | anti-YOD1 antibody

YOD1 Antibody (C-term)

Gene Names
YOD1; DUBA8; OTUD2; PRO0907
Reactivity
Human (Predicted Reactivity: Chicken, Zebrafish)
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
YOD1, Antibody; YOD1 Antibody (C-term); Ubiquitin thioesterase OTU1; DUBA-8; HIV-1-induced protease 7; HIN-7; HsHIN7; OTU domain-containing protein 2; YOD1; DUBA8; HIN7; OTUD2; anti-YOD1 antibody
Ordering
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Chicken, Zebrafish)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This YOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-347 amino acids from the C-terminal region of human YOD1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.3 (varies by lot)
Sequence Positions
319-347
Sequence Length
304
Applicable Applications for anti-YOD1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of YOD1 Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

product-image-AAA283648_IF11.jpg IF (Immunofluorescence) (Confocal immunofluorescent analysis of YOD1 Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohiostchemistry)

(YOD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YOD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

product-image-AAA283648_IHC13.jpg IHC (Immunohiostchemistry) (YOD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YOD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(YOD1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the YOD1 antibody detected the YOD1 protein (arrow).)

product-image-AAA283648_WB15.jpg WB (Western Blot) (YOD1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the YOD1 antibody detected the YOD1 protein (arrow).)
Related Product Information for anti-YOD1 antibody
Deubiquitinating enzymes (DUBs; see MIM 603478) are
proteases that specifically cleave ubiquitin (MIM 191339) linkages,
negating the action of ubiquitin ligases. DUBA8 belongs to a DUB
subfamily characterized by an ovarian tumor (OTU) domain.[supplied
by OMIM].
Product Categories/Family for anti-YOD1 antibody
References
Ernst, R., et al. Mol. Cell 36(1):28-38(2009)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38322
NCBI Official Full Name
ubiquitin thioesterase OTU1 isoform 2
NCBI Official Synonym Full Names
YOD1 deubiquitinase
NCBI Official Symbol
YOD1
NCBI Official Synonym Symbols
DUBA8; OTUD2; PRO0907
NCBI Protein Information
ubiquitin thioesterase OTU1
UniProt Protein Name
Ubiquitin thioesterase OTU1
UniProt Gene Name
YOD1
UniProt Synonym Gene Names
DUBA8; HIN7; OTUD2; HIN-7; HsHIN7
UniProt Entry Name
OTU1_HUMAN

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Product Notes

The YOD1 yod1 (Catalog #AAA283648) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 319-347. The YOD1 Antibody (C-term) reacts with Human (Predicted Reactivity: Chicken, Zebrafish) and may cross-react with other species as described in the data sheet. AAA Biotech's YOD1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the YOD1 yod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YOD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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