Mouse HLA-Pan Antibody | anti-HLA-Pan antibody

HLA-Pan (MHC II)

Cat. Num. AAA215333

• Gene Names: HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
• Reactivity: Human. Others not known
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: HLA-Pan; N/A; HLA-Pan (MHC II); HLA-DPB1; HLA-DQA1; HLA-DQB1; HLA-DRA; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5; anti-HLA-Pan antibody

• Host: Mouse
• Reactivity: Human. Others not known
• Isotype: IgG1, kappa
• Clone Number: rHLA-Pan/3475
• Specificity: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 258

• Applicable Applications for anti-HLA-Pan antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
• Positive Control: Raji cells. Tonsil or lymph node.
• Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cells
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 6p21.3 (HLA-DP & HLA-DR)
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide - store at 2 to 8°C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Immunofluorescence Analysis of Human Raji cells with HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).)

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of Human Raji cells using HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).)

SDS-PAGE

(SDS-PAGE Analysis Purified HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475).)

References

1. Gatter et al. 1982. Semin Oncol. 9(4):517-25. 2. Gatter et al. 1982. J Clin Pathol. 35(11):1253-67.

NCBI and Uniprot Product Information

• NCBI GI #: 122263
• NCBI GeneID: 3115
• UniProt Accession #: P04440; P01908; P01909; P01920; P01903
• Molecular Weight: 33-35kDa
• NCBI Official Full Name: HLA class II histocompatibility antigen, DP beta 1 chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DP beta 1
• NCBI Official Symbol: HLA-DPB1
• NCBI Official Synonym Symbols: DPB1; HLA-DP; HLA-DPB; HLA-DP1B
• NCBI Protein Information: HLA class II histocompatibility antigen, DP beta 1 chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DP beta 1 chain
• UniProt Gene Name: HLA-DPB1
• UniProt Synonym Gene Names: HLA-DP1B
• UniProt Entry Name: DPB1_HUMAN