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FCM/FACS (Flow Cytometry)
(Flow cytometry intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5ug/ml, Streptavidin PE).)
Mouse anti-Human IL-2 Antibody | anti-IL-2 antibody
Anti-Hu IL-2 Biotin
Gene Names
IL2; IL-2; TCGF; lymphokine
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Western Blot
Purity
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Synonyms
IL-2; N/A; Anti-Hu IL-2 Biotin; interleukin 2; Interleukin 2; anti-IL-2 antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG2b kappa
Clone Number
35C3
Specificity
The mouse monoclonal antibody 35C3 recognizes human interleukin 2 (IL-2; secreted or intracellular).
Purity/Purification
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Form/Format
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IL-2 antibody
IP (Immunoprecipitation), ELISA, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
recombinant human IL-2
Conjugation
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
FCM/FACS (Flow Cytometry)
(Flow cytometry intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5ug/ml, Streptavidin PE).)
FCM/FACS (Flow Cytometry)
(Flow cytometry intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5ug/ml, Streptavidin PE).)
FCM/FACS (Flow Cytometry)
(Separation of human CD3 positive IL-2 positive lymphocytes (red-filled) from CD3 negative IL-2 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5ug/ml, Streptavidin PE).)
FCM/FACS (Flow Cytometry)
(Separation of human CD3 positive IL-2 positive lymphocytes (red-filled) from CD3 negative IL-2 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5ug/ml, Streptavidin PE).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD4 (MEM-241) APC antibody (10ul reagent/100ul of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5ug/ml, Streptavidin PE).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD4 (MEM-241) APC antibody (10ul reagent/100ul of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5ug/ml, Streptavidin PE).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) Pacific Blue antibody (4ul reagent/100ul of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5ug/ml, Streptavidin PE).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) Pacific Blue antibody (4ul reagent/100ul of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5ug/ml, Streptavidin PE).)
ELISA
(ELISA analysis of human IL-2 (100 ng/ml) using biotin-conjugated mouse monoclonal antibody 35C3 in five different concentrations (0.0016 - 1ug/ml).)
ELISA
(ELISA analysis of human IL-2 (100 ng/ml) using biotin-conjugated mouse monoclonal antibody 35C3 in five different concentrations (0.0016 - 1ug/ml).)
Related Product Information for anti-IL-2 antibody
IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However, IL-2 also stimulates growth and differentiation of B cells, NK cells, monocytes and other cell types, such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,628 Da
NCBI Official Full Name
interleukin-2
NCBI Official Synonym Full Names
interleukin 2
NCBI Official Symbol
IL2
NCBI Official Synonym Symbols
IL-2; TCGF; lymphokine
NCBI Protein Information
interleukin-2; aldesleukin; T cell growth factor; involved in regulation of T-cell clonal expansion
UniProt Protein Name
Interleukin-2
UniProt Gene Name
IL2
UniProt Synonym Gene Names
IL-2; TCGF
UniProt Entry Name
IL2_HUMAN
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Product Notes
The IL-2 il2 (Catalog #AAA129328) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu IL-2 Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IL-2 il2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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