IF (Immunofluorescence)
(Figure 3: Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Mouse GSK3B Antibody | anti-GSK3B antibody
GSK3B Antibody
Reactivity
Human, Rat, Mouse, Monkey
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
GSK3B; N/A; GSK3B Antibody; GSK-3beta; GSK3-beta; anti-GSK3B antibody
Host
Mouse
Reactivity
Human, Rat, Mouse, Monkey
Isotype
IgG1
Clone Number
3D10
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
420
Applicable Applications for anti-GSK3B antibody
ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Purified recombinant fragment of human GSK3B expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.
IF (Immunofluorescence)
(Figure 3: Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IF (Immunofluorescence)
(Figure 3: Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry)
(Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3B mouse mAb with DAB staining.)
IHC (Immunohiostchemistry)
(Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3B mouse mAb with DAB staining.)
WB (Western Blot)
(Figure 1: Western blot analysis using GSK3B mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysate.)
WB (Western Blot)
(Figure 1: Western blot analysis using GSK3B mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysate.)
Related Product Information for anti-GSK3B antibody
Glycogen synthase kinase 3 (GSK-3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK-3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK-3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK-3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK-3 are ubiquitously expressed, although particularly high levels of GSK-3beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK-3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines.
Product Categories/Family for anti-GSK3B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46kDa
NCBI Official Full Name
glycogen synthase kinase-3 beta isoform 2
NCBI Official Synonym Full Names
glycogen synthase kinase 3 beta
NCBI Official Symbol
GSK3B
NCBI Protein Information
glycogen synthase kinase-3 beta
UniProt Protein Name
Glycogen synthase kinase-3 beta
UniProt Gene Name
GSK3B
UniProt Synonym Gene Names
GSK-3 beta
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Product Notes
The GSK3B gsk3b (Catalog #AAA108743) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GSK3B Antibody reacts with Human, Rat, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's GSK3B can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GSK3B gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3B, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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