IF (Immunofluorescence)
(Immunofluorescence staining of fixed U937 cells with anti-CCR5 antibody ST6 (AAA72266). Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix cover-slips stained with the chimeric rabbit IgG version of ST6 (AAA72266-23.0) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72266-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Mouse CCR5 Antibody | anti-CCR5 antibody
Anti-CCR5 [ST6], Mouse IgG1
Gene Names
CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
Reactivity
Human, rhesus macaque
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
CCR5; N/A; Anti-CCR5 [ST6], Mouse IgG1; Anti-CCR5 [ST6]; CD195; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CHEMR13; HIV-1 fusion coreceptor; B8LFP0; anti-CCR5 antibody
Host
Mouse
Reactivity
Human, rhesus macaque
Isotype
Mouse IgG1, Kappa
Clone Number
ST6
Specificity
This antibody is specific for the YTSE region of the N-terminal extracellular domain of human CCR5.It also binds to rhesus CCR5.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CCR5 antibody
SPR (Surface Plasmon Resonance), ELISA, FCM/FACS (Flow Cytometry)
Immunogen
This antibody was isolated from a phage displayed antibody fragment library. The library was generated from a New Zealand White rabbit immunised with purified CCR5-N-GST protein, and four rounds of panning were completed against immobilised CCR5-N-MBP antigen.
Origin Pub PMID
10969070
Chimeric Use Statement
This chimeric mouse antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of fixed U937 cells with anti-CCR5 antibody ST6 (AAA72266). Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix cover-slips stained with the chimeric rabbit IgG version of ST6 (AAA72266-23.0) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72266-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed U937 cells with anti-CCR5 antibody ST6 (AAA72266). Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix cover-slips stained with the chimeric rabbit IgG version of ST6 (AAA72266-23.0) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72266-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western Blot using anti-CCR5 antibody ST6 (AAA72266). K562 cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of ST6 (AAA72266-23.0) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-CCR5 antibody ST6 (AAA72266). K562 cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of ST6 (AAA72266-23.0) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CCR5 antibody ST6 (AAA72266). Paraformaldehyde fixed U937 cells were stained with anti-unknown specificity antibody or the rabbit IgG version of ST6 (AAA72266-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CCR5 antibody ST6 (AAA72266). Paraformaldehyde fixed U937 cells were stained with anti-unknown specificity antibody or the rabbit IgG version of ST6 (AAA72266-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-CCR5 antibody
When expressed intracellularly, this antibody is able to inhibit the surface expression of human CCR5, conferring T-lymphocytes with immunity to CCR5-dependent HIV-1 infection (Steinberger et al, 2000). Humanised ST6 (ST6/34) also has the same depleting effect (Steinberger et al, 2000). The high affinity of this antibody for CCR5-N-MBP has been confirmed in surface plasmon resonance analysis (Steinberg et al, 2000). This antibody has been used to stain CCR5-expressing cells in flow cytometry, with no staining of control cells observed (Steinberger et al, 2000).
Product Categories/Family for anti-CCR5 antibody
References
Steinberger et al. Generation and Characterization of a Recombinant Human CCR5-specific Antibody: A PHAGE DISPLAY APPROACH FOR RABBIT ANTIBODY HUMANIZATION J Biol Chem. 2000 Nov 17;275(46):36073-8. PMID:10969070
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,524 Da
NCBI Official Full Name
C-C chemokine receptor type 5
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 5 (gene/pseudogene)
NCBI Official Symbol
CCR5
NCBI Official Synonym Symbols
CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
NCBI Protein Information
C-C chemokine receptor type 5; chemr13; HIV-1 fusion coreceptor; chemokine receptor CCR5; C-C motif chemokine receptor 5 A159A
UniProt Protein Name
C-C chemokine receptor type 5
UniProt Gene Name
CCR5
UniProt Synonym Gene Names
CMKBR5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5
UniProt Entry Name
CCR5_HUMAN
Similar Products
Product Notes
The CCR5 ccr5 (Catalog #AAA72266) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CCR5 [ST6], Mouse IgG1 reacts with Human, rhesus macaque and may cross-react with other species as described in the data sheet. AAA Biotech's CCR5 can be used in a range of immunoassay formats including, but not limited to, SPR (Surface Plasmon Resonance), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CCR5 ccr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR5, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.