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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2750-2800 of 96812 product results
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of PC-3 cells using anti-RFXANK antibody (AAA127488).Overlay histogram showing PC-3 cells stained with AAA127488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RFXANK Antibody (AAA127488, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RFXANK, Polyclonal Antibody (Cat# AAA127488)
Full Name
Anti-RFXANK Antibody Picoband
Gene Names
RFXANK; BLS; RFX-B; ANKRA1; F14150_1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of 293T cells using anti-NSFL1C antibody (AAA127501).Overlay histogram showing 293T cells stained with AAA127501 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NSFL1C Antibody (AAA127501, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NSFL1C, Polyclonal Antibody (Cat# AAA127501)
Full Name
Anti-NSFL1C Antibody Picoband
Gene Names
NSFL1C; P47; UBX1; UBXD10; UBXN2C; dJ776F14.1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-SPATA7 antibody (AAA127514).Overlay histogram showing HepG2 cells stained with AAA127514 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPATA7 Antibody (AAA127514, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
SPATA7, Polyclonal Antibody (Cat# AAA127514)
Full Name
Anti-SPATA7 Antibody Picoband
Gene Names
SPATA7; HSD3; LCA3; HSD-3.1; HEL-S-296
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U251 cells using anti-CAPN5 antibody (AAA127518).Overlay histogram showing U251 cells stained with AAA127518 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CAPN5 Antibody (AAA127518, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CAPN5, Polyclonal Antibody (Cat# AAA127518)
Full Name
Anti-CAPN5 Antibody Picoband
Gene Names
CAPN5; VRNI; ADNIV; HTRA3; nCL-3
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-ADAM2 antibody (AAA127519).Overlay histogram showing MCF-7 cells stained with AAA127519 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAM2 Antibody (AAA127519, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ADAM2, Polyclonal Antibody (Cat# AAA127519)
Full Name
Anti-ADAM2 Antibody Picoband
Gene Names
ADAM2; CT15; FTNB; PH30; CRYN1; CRYN2; PH-30b; PH30-beta
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-PIGV antibody (AAA127540).Overlay histogram showing U937 cells stained with AAA127540 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIGV Antibody (AAA127540, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PIGV, Polyclonal Antibody (Cat# AAA127540)
Full Name
Anti-PIGV Antibody Picoband
Gene Names
PIGV; PIG-V; HPMRS1; GPI-MT-II
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A549 cells using anti-HIBADH antibody (AAA127543).Overlay histogram showing A549 cells stained with AAA127543 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIBADH Antibody (AAA127543, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HIBADH, Polyclonal Antibody (Cat# AAA127543)
Full Name
Anti-HIBADH Antibody Picoband
Gene Names
HIBADH; NS5ATP1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of PC-3 cells using anti-ITM2C antibody (AAA127549).Overlay histogram showing PC-3 cells stained with AAA127549 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ITM2C Antibody (AAA127549, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
ITM2C, Polyclonal Antibody (Cat# AAA127549)
Full Name
Anti-ITM2C Antibody Picoband
Gene Names
ITM2C; E25; BRI3; E25C; ITM3; BRICD2C
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U937 cells using anti-NDUFA5 antibody (AAA127555).Overlay histogram showing U937 cells stained with AAA127555 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA5 Antibody (AAA127555, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NDUFA5, Polyclonal Antibody (Cat# AAA127555)
Full Name
Anti-NDUFA5 Antibody Picoband
Gene Names
NDUFA5; B13; NUFM; UQOR13; CI-13kB; CI-13KD-B
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HepG2 cells using anti-SPECC1L antibody (AAA127568).Overlay histogram showing HepG2 cells stained with AAA127568 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPECC1L Antibody (AAA127568, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
SPECC1L, Polyclonal Antibody (Cat# AAA127568)
Full Name
Anti-SPECC1L Antibody Picoband
Gene Names
SPECC1L; CYTSA; OBLFC1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-STAP-1/STAP1 antibody (AAA127569).Overlay histogram showing U937 cells stained with AAA127569 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAP-1/STAP1 Antibody (AAA127569, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
STAP-1/STAP1, Polyclonal Antibody (Cat# AAA127569)
Full Name
Anti-STAP-1/STAP1 Antibody Picoband
Gene Names
STAP1; BRDG1; STAP-1
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HEL cells using anti-MYLK3 antibody (AAA127571).Overlay histogram showing HEL cells stained with AAA127571 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYLK3 Antibody (AAA127571, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MYLK3, Polyclonal Antibody (Cat# AAA127571)
Full Name
Anti-MYLK3 Antibody Picoband
Gene Names
MYLK3; MLCK; MLCK2; caMLCK
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of K562 cells using anti- GATA1 antibody (AAA125545).Overlay histogram showing K562 cells stained with AAA125545 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- GATA1 Antibody (AAA125545, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GATA1, Polyclonal Antibody (Cat# AAA125545)
Full Name
Anti-GATA1 Antibody
Gene Names
GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; XLANP; XLTDA; GATA-1
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A549 cells using anti-TRPA1/TSA antibody (AAA125546).Overlay histogram showing A549 cells stained with AAA125546 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPA1/TSA Antibody (AAA125546, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TRPA1/TSA, Polyclonal Antibody (Cat# AAA125546)
Full Name
Anti-TRPA1/TSA Antibody
Gene Names
TRPA1; FEPS; ANKTM1
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of SiHa cells using anti-BCRP/ABCG2 antibody (AAA125549).Overlay histogram showing SiHa cells stained with AAA125549 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCRP/ABCG2 Antibody (AAA125549,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
BCRP/ABCG2, Polyclonal Antibody (Cat# AAA125549)
Full Name
Anti-BCRP/ABCG2 Antibody
Gene Names
ABCG2; MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; CDw338; UAQTL1; EST157481
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 3. IF analysis of XPC using anti- XPC antibody (AAA125551).XPC was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-XPC Antibody (AAA125551) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
XPC, Polyclonal Antibody (Cat# AAA125551)
Full Name
Anti-XPC Antibody
Gene Names
XPC; XP3; RAD4; XPCC; p125
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U20S cells using anti-FLT1 antibody (AAA125557).Overlay histogram showing U20S cells stained with AAA125557 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FLT1 Antibody (AAA125557, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FLT1, Polyclonal Antibody (Cat# AAA125557)
Full Name
Anti-FLT1 Antibody
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of SiHa cells using anti-Poliovirus Receptor/PVR antibody (AAA125567).Overlay histogram showing SiHa cells stained with AAA125567 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Poliovirus Receptor/PVR Antibody (AAA125567, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Poliovirus Receptor/PVR, Polyclonal Antibody (Cat# AAA125567)
Full Name
Anti-Poliovirus Receptor/PVR Antibody
Gene Names
PVR; PVS; HVED; CD155; NECL5; TAGE4; Necl-5
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U20S cells using anti-Thrombospondin/THBS1 antibody (AAA125568).Overlay histogram showing U20S cells stained with AAA125568 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Thrombospondin/THBS1 Antibody (AAA125568, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Thrombospondin/THBS1, Polyclonal Antibody (Cat# AAA125568)
Full Name
Anti-Thrombospondin/THBS1 Antibody
Gene Names
THBS1; TSP; THBS; TSP1; TSP-1; THBS-1
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of CD34 using anti-CD34 antibody (AAA125577).CD34 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (AAA125577) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CD34, Polyclonal Antibody (Cat# AAA125577)
Full Name
Anti-CD34 Antibody
Reactivity
Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of THP-1 cells using anti-NNT antibody (AAA125578).Overlay histogram showing THP-1 cells stained with AAA125578 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NNT Antibody (AAA125578,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NNT, Polyclonal Antibody (Cat# AAA125578)
Full Name
Anti-NNT Antibody
Gene Names
NNT; GCCD4
Reactivity
Human, Mouse, Monkey, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 3. IF analysis of ROCK2 using anti- ROCK2 antibody (AAA125586).ROCK2 was detected in immunocytochemical section of Hep cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- ROCK2 Antibody (AAA125586) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
ROCK2, Polyclonal Antibody (Cat# AAA125586)
Full Name
Anti-ROCK2 Antibody
Gene Names
ROCK2; ROCK-II
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U20S cells using anti-MUC2 antibody (AAA125594).Overlay histogram showing U20S cells stained with AAA125594 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MUC2 Antibody (AAA125594,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MUC2, Polyclonal Antibody (Cat# AAA125594)
Full Name
Anti-MUC2 Antibody
Gene Names
MUC2; MLP; SMUC; MUC-2
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of CD30/TNFRSF8 using anti-CD30/TNFRSF8 antibody (AAA125595).CD30/TNFRSF8 was detected in paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD30/TNFRSF8 Antibody (AAA125595) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CD30/TNFRSF8, Polyclonal Antibody (Cat# AAA125595)
Full Name
Anti-CD30/TNFRSF8 Antibody
Gene Names
TNFRSF8; CD30; Ki-1; D1S166E
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A549 cells using anti-TRAF1 antibody (AAA125606).Overlay histogram showing A549 cells stained with AAA125606 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAF1 Antibody (AAA125606, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TRAF1, Polyclonal Antibody (Cat# AAA125606)
Full Name
Anti-TRAF1 Antibody
Gene Names
TRAF1; EBI6; MGC:10353
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of Raji cells using anti-Integrin alpha V/ITGAV antibody (AAA125610).Overlay histogram showing Raji cells stained with AAA125610 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha V/ITGAV Antibody (AAA125610,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Integrin alpha V/ITGAV, Polyclonal Antibody (Cat# AAA125610)
Full Name
Anti-Integrin alpha V/ITGAV Antibody
Gene Names
ITGAV; CD51; MSK8; VNRA; VTNR
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 5. IF analysis of Claudin 1/CLDN1 using anti- Claudin 1/CLDN1 antibody (AAA125612).Claudin 1/CLDN1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti- Claudin 1/CLDN1 Antibody (AAA125612) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Claudin 1/CLDN1, Polyclonal Antibody (Cat# AAA125612)
Full Name
Anti-Claudin 1/CLDN1 Antibody
Gene Names
CLDN1; CLD1; SEMP1; ILVASC
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-CYP7A1 antibody (AAA125614).Overlay histogram showing U87 cells stained with AAA125614 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP7A1 Antibody (AAA125614, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CYP7A1, Polyclonal Antibody (Cat# AAA125614)
Full Name
Anti-CYP7A1 Antibody
Gene Names
CYP7A1; CP7A; CYP7; CYPVII
Reactivity
Human, Mouse, Rat, Monkey
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 4. IF analysis of IL37 using anti- IL37 antibody (AAA125697).IL37 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- IL37 Antibody (AAA125697) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IL37, Polyclonal Antibody (Cat# AAA125697)
Full Name
Anti-IL37 Antibody
Gene Names
IL37; FIL1; FIL1Z; IL-1H; IL-37; IL1F7; IL1H4; IL-1F7; IL-1H4; IL1RP1; IL-1RP1; FIL1(ZETA)
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-CYP11B1/C11B2/CYP11B2 antibody (AAA125703).Overlay histogram showing 293T cells stained with AAA125703 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP11B1/C11B2/CYP11B2 Antibody (AAA125703,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CYP11B1/C11B2/CYP11B2, Polyclonal Antibody (Cat# AAA125703)
Full Name
Anti-CYP11B1/C11B2/CYP11B2 Antibody
Gene Names
CYP11B1; FHI; CPN1; CYP11B; P450C11
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 2. Western blot analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (AAA125723).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat PC-12 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Claudin 3/CLDN3 antigen affinity purified polyclonal antibody (Catalog # AAA125723) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Claudin 3/CLDN3 at approximately 23KD. The expected band size for Claudin 3/CLDN3 is at 23KD.)
Claudin 3/CLDN3, Polyclonal Antibody (Cat# AAA125723)
Full Name
Anti-Claudin 3/CLDN3 Antibody
Gene Names
Cldn3; mRVP1; Cpetr2; AI182374
Reactivity
Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U20S cells using anti-LXR beta/NER/NR1H2 antibody (AAA125726).Overlay histogram showing U20S cells stained with AAA125726 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LXR beta/NER/NR1H2 Antibody (AAA125726, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
LXR beta/NER/NR1H2, Polyclonal Antibody (Cat# AAA125726)
Full Name
Anti-LXR beta/NER/NR1H2 Antibody
Gene Names
NR1H2; NER; UNR; LXRB; LXR-b; NER-I; RIP15
Reactivity
Human, Mouse
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of Hela cells using anti-SLC22A3 antibody (AAA125731).Overlay histogram showing Hela cells stained with AAA125731 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC22A3 Antibody (AAA125731, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SLC22A3, Polyclonal Antibody (Cat# AAA125731)
Full Name
Anti-SLC22A3 Antibody
Gene Names
SLC22A3; EMT; EMTH; OCT3
Reactivity
Human, Mouse
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of LOC134147/CMBL using anti-LOC134147/CMBL antibody (AAA125733).LOC134147/CMBL was detected in paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LOC134147/CMBL Antibody (AAA125733) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
LOC134147/CMBL, Polyclonal Antibody (Cat# AAA125733)
Full Name
Anti-LOC134147/CMBL Antibody
Gene Names
CMBL; JS-1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U20S cells using anti-Kir6. 1/KCNJ8 antibody (AAA125737).Overlay histogram showing U20S cells stained with AAA125737 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Kir6. 1/KCNJ8 Antibody (AAA125737, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Kir6. 1/KCNJ8, Polyclonal Antibody (Cat# AAA125737)
Full Name
Anti-Kir6. 1/KCNJ8 Antibody
Gene Names
KCNJ8; KIR6.1; uKATP-1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of ACADS/SCAD using anti-ACADS/SCAD antibody (AAA125738).ACADS/SCAD was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ACADS/SCAD Antibody (AAA125738) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
ACADS/SCAD, Polyclonal Antibody (Cat# AAA125738)
Full Name
Anti-ACADS/SCAD Antibody
Gene Names
ACADS; SCAD; ACAD3
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 4. IF analysis of CTHRC1 using anti-CTHRC1 antibody (AAA125741).CTHRC1 was detected in immunocytochemical section of HPEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-CTHRC1 Antibody (AAA125741) overnight at 4 degree C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
CTHRC1, Polyclonal Antibody (Cat# AAA125741)
Full Name
Anti-CTHRC1 Antibody
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of Hela cells using anti-Tubulin beta antibody (AAA125748).Overlay histogram showing Hela cells stained with AAA125748 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tubulin beta Antibody (AAA125748,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Tubulin beta, Polyclonal Antibody (Cat# AAA125748)
Full Name
Anti-Tubulin beta Antibody
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of HEPA1-6 cells using anti-GILT/IFI30 antibody (AAA125756).Overlay histogram showing HEPA1-6 cells stained with AAA125756 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GILT/IFI30 Antibody (AAA125756, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GILT/IFI30, Polyclonal Antibody (Cat# AAA125756)
Full Name
Anti-GILT/IFI30 Antibody
Gene Names
Ifi30; GILT; IP30
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-SEC23B antibody (AAA125762).Overlay histogram showing SiHa cells stained with AAA125762 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC23B Antibody (AAA125762, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SEC23B, Polyclonal Antibody (Cat# AAA125762)
Full Name
Anti-SEC23B Antibody
Gene Names
SEC23B; CDAII; CDAN2; CDA-II; HEMPAS
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of PC-3 cells using anti-K Cadherin/CDH6 antibody (AAA125770).Overlay histogram showing PC-3 cells stained with AAA125770 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-K Cadherin/CDH6 Antibody (AAA125770, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
K Cadherin/CDH6, Polyclonal Antibody (Cat# AAA125770)
Full Name
Anti-K Cadherin/CDH6 Antibody
Gene Names
CDH6; CAD6; KCAD
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 4. IF analysis of PTCH2 using anti- PTCH2 antibody (AAA125771).PTCH2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-PTCH2 Antibody (AAA125771) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
PTCH2, Polyclonal Antibody (Cat# AAA125771)
Full Name
Anti-PTCH2 Antibody
Gene Names
PTCH2; PTC2
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-MT2-MMP/MMP15 antibody (AAA125772).Overlay histogram showing CACO-2 cells stained with AAA125772 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MT2-MMP/MMP15 Antibody (AAA125772, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MT2-MMP/MMP15, Polyclonal Antibody (Cat# AAA125772)
Full Name
Anti-MT2-MMP/MMP15 Antibody
Gene Names
MMP15; MTMMP2; SMCP-2; MT2-MMP
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of ErbB 4 using anti-ErbB 4 antibody (AAA124563).ErbB 4 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ErbB 4 Antibody (AAA124563) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
ErbB 4, Polyclonal Antibody (Cat# AAA124563)
Full Name
Anti-ErbB 4 Picoband antibody
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of Jurkat cells using anti-NFATC1 antibody (AAA124564).Overlay histogram showing Jurkat cells stained with AAA124564 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFATC1 Antibody (AAA124564,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NFAT2, Polyclonal Antibody (Cat# AAA124564)
Full Name
Anti-NFAT2 Picoband Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of NADPH oxidase 4 using anti-NADPH oxidase 4 antibody (AAA124570).NADPH oxidase 4 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NADPH oxidase 4 Antibody (AAA124570) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
NADPH oxidase 4, Polyclonal Antibody (Cat# AAA124570)
Full Name
Anti-NADPH oxidase 4 Picoband antibody
Gene Names
NOX4; KOX; KOX-1; RENOX
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of Beta 2 Microglobulin using anti- Beta 2 Microglobulin antibody (AAA124573).Beta 2 Microglobulin was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Beta 2 Microglobulin Antibody (AAA124573) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Beta 2 Microglobulin, Polyclonal Antibody (Cat# AAA124573)
Full Name
Anti-Beta 2 Microglobulin Picoband Antibody
Gene Names
B2M; IMD43
Reactivity
Human
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of DGCR8 using anti-DGCR8 antibody (AAA124576).DGCR8 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DGCR8 Antibody (AAA124576) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
DGCR8, Polyclonal Antibody (Cat# AAA124576)
Full Name
Anti-DGCR8 Picoband antibody
Gene Names
DGCR8; Gy1; pasha; DGCRK6; C22orf12
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of Cytochrome P450 2D6 using anti- Cytochrome P450 2D6 antibody (AAA124578).Cytochrome P450 2D6 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Cytochrome P450 2D6 Antibody (AAA124578) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Cytochrome P450 2D6, Polyclonal Antibody (Cat# AAA124578)
Full Name
Anti-Cytochrome P450 2D6 Picoband Antibody
Gene Names
CYP2D6; CPD6; CYP2D; CYP2DL1; CYPIID6; P450C2D; P450DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; P450-DB1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(IHC analysis of PON1 using anti-PON1 antibody (AAA124579). PON1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PON1 Antibody (AAA124579) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
PON1/Paraoxonase 1, Polyclonal Antibody (Cat# AAA124579)
Full Name
Anti-PON1/Paraoxonase 1 Picoband Antibody
Gene Names
Pon1; Pon
Reactivity
Mouse
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. This ability to bind multiple epitopes is what makes polyclonal antibodies highly sensitive, as explained in our detailed guide on polyclonal antibodies and why they matter.
As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in our catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.